PMID- 7705186
OWN - NLM
STAT- MEDLINE
DCOM- 19950511
LR  - 20071114
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 19
IP  - 1
DP  - 1995 Jan 1
TI  - Semiautomated DNA probe mapping using digital imaging microscopy: II. System 
      performance.
PG  - 60-9
AB  - This paper describes an evaluation of a semiautomated, multicolor image-analysis 
      system to map cloned probes along metaphase chromosomes. Mapping with this system 
      consists of fluorescence in situ hybridization (FISH) for probe localization, 
      automatic acquisition of multicolor images showing total chromosomal DNA and 
      probe location(s), and automatic determination of the fractional locations of the 
      probes along the chromosomes relative to the short arm telomere (FLpter). The 
      system was evaluated by mapping ten phage and ten cosmid probes previously mapped 
      to chromosome 3 with other procedures. The standard deviations of FLpter 
      measurements averaged 3.4 Mb and 2.6 Mb for phage and cosmid probes, 
      respectively. With this variation, the order of two probes mapped in separate 
      hybridizations could be determined with 95% confidence when their separation was 
      greater than 2.5 Mb. In all cases, the probe locations and order were consistent 
      with previous mapping data. FLpter values were converted to band locations using 
      measurements of the band locations made using digital imaging microscopy. This 
      proved superior to conversions made using ISCN ideograms.
FAU - Sakamoto, M
AU  - Sakamoto M
AD  - Division of Molecular Cytometry, University of California, San Francisco 
      94143-0808, USA.
FAU - Pinkel, D
AU  - Pinkel D
FAU - Mascio, L
AU  - Mascio L
FAU - Sudar, D
AU  - Sudar D
FAU - Peters, D
AU  - Peters D
FAU - Kuo, W L
AU  - Kuo WL
FAU - Yamakawa, K
AU  - Yamakawa K
FAU - Nakamura, Y
AU  - Nakamura Y
FAU - Drabkin, H
AU  - Drabkin H
FAU - Jericevic, Z
AU  - Jericevic Z
AU  - et al.
LA  - eng
GR  - CA 45919/CA/NCI NIH HHS/United States
GR  - HD 17665/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (DNA Probes)
SB  - IM
MH  - Bacteriophages
MH  - Chromosome Mapping/*methods
MH  - Cosmids
MH  - DNA Probes
MH  - Humans
MH  - Image Processing, Computer-Assisted/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy, Fluorescence/instrumentation/*methods
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1002/cyto.990190108 [doi]
PST - ppublish
SO  - Cytometry. 1995 Jan 1;19(1):60-9. doi: 10.1002/cyto.990190108.