PMID- 7705946
OWN - NLM
STAT- MEDLINE
DCOM- 19950510
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 61
IP  - 2
DP  - 1995 Apr 10
TI  - Sensitive detection of numerical and structural aberrations of chromosome 1 in 
      neuroblastoma by interphase fluorescence in situ hybridization. Comparison with 
      restriction fragment length polymorphism and conventional cytogenetic analyses.
PG  - 185-91
AB  - Chromosome I abnormalities are indicators of prognosis in neuroblastoma (NB) but 
      are not yet routinely exploited because conventional methods are technically 
      demanding. We evaluated the pertinence of interphase cytogenetics fluorescence in 
      situ hybridization (FISH) for the analysis of chromosome I in NB, compared with 
      conventional methods. Deletion of Ip was detected in 8 of 9 cell lines analyzed 
      by both FISH and restriction fragment length polymorphism (RFLP), but was 
      evidenced in only 2 cases by conventional cytogenetics, painting analysis being 
      required to reveal the other cases. The chromosome I number evaluated by FISH 
      reflected the total chromosome modal number obtained by cytogenetics. 
      Twenty-eight specimens obtained from ultrasound-guided punctures, surgical 
      biopsies of the primary tumor and bone-marrow aspirates were studied by FISH on 
      frozen cytocentrifuged smears; 12 had a chromosome I trisomy and 16 a disomy. 
      Requirements for a reliable control analysis of Ip deletion by RFLP were met in 
      only 23 cases. The retention of 2 alleles was observed in 15 cases and Ip 
      deletion in 7, by both techniques. In one case, an interstitial deletion of Ip 
      was evidenced only by RFLP, and one of 5 cases analyzed only by FISH had a Ip 
      deletion. Although FISH might be improved by using additional probes, it presents 
      major advantages for routine exploitation. Determining Ip deletion in individual 
      cells makes it possible to analyze small and heterogeneous tumoral specimens; the 
      technique requires only a few hours and can easily be standardized in 
      non-specialized laboratories. The number of chromosome I homologues per cell 
      might serve as a rapid screening for ploidy.
FAU - Combaret, V
AU  - Combaret V
AD  - Department of Radiology, Centre Leon Berard, Lyon, France.
FAU - Turc-Carel, C
AU  - Turc-Carel C
FAU - Thiesse, P
AU  - Thiesse P
FAU - Rebillard, A C
AU  - Rebillard AC
FAU - Frappaz, D
AU  - Frappaz D
FAU - Haus, O
AU  - Haus O
FAU - Philip, T
AU  - Philip T
FAU - Favrot, M C
AU  - Favrot MC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
SB  - IM
MH  - Biopsy
MH  - Blotting, Southern
MH  - Bone Marrow/pathology
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 1
MH  - Cytogenetics/*methods
MH  - Fluorescent Antibody Technique
MH  - Gene Deletion
MH  - Heterozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Neuroblastoma/*genetics/pathology
MH  - Polymorphism, Restriction Fragment Length
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tumor Cells, Cultured
EDAT- 1995/04/10 00:00
MHDA- 1995/04/10 00:01
CRDT- 1995/04/10 00:00
PHST- 1995/04/10 00:00 [pubmed]
PHST- 1995/04/10 00:01 [medline]
PHST- 1995/04/10 00:00 [entrez]
AID - 10.1002/ijc.2910610208 [doi]
PST - ppublish
SO  - Int J Cancer. 1995 Apr 10;61(2):185-91. doi: 10.1002/ijc.2910610208.