PMID- 7707284
OWN - NLM
STAT- MEDLINE
DCOM- 19950510
LR  - 20190724
IS  - 0022-4251 (Print)
IS  - 0022-4251 (Linking)
VI  - 103
IP  - 1
DP  - 1995 Jan
TI  - Binding of pig sperm receptor in the zona pellucida to the boar sperm acrosome.
PG  - 1-8
AB  - Pig zona pellucida (ZP) contains three families of glycoproteins: PZP2, PZP3 
      alpha and PZP3 beta. PZP3 alpha mediates the binding of the ZP to spermatozoa. In 
      this study, the binding site of pig ZP on boar spermatozoa and the zona-binding 
      proteins of boar spermatozoa were studied using chemically modified zona 
      glycoproteins or anti-pig ZP antiserum. Endo-beta-galactosidase-digested PZP3 
      alpha (E beta G-PZP3 alpha), which is deficient in sulfated 
      N-acetylpolyactosamine, as well as solubilized ZP, bound to the acrosomal region 
      of acrosome-damaged or partially acrosome-reacted spermatozoa. However, they did 
      not bind to acrosome-intact or fully acrosome-reacted spermatozoa. Solubilized ZP 
      did bind to the acrosomal cap released upon acrosome reaction. In western blot 
      analyses, E beta G-PZP3 alpha bound to the sperm proteins with molecular masses 
      similar to proacrosin-acrosin and the binding was inhibited by fucoidan and 
      anti-pig acrosin antiserum. These results suggest that the binding site of 
      solubilized pig ZP and E beta G-PZP3 alpha on spermatozoa is located mainly in 
      the acrosomal matrix and on the membranous compartments in the acrosome and 
      suggest that E beta G-PZP3 alpha binds to proacrosin-acrosin. The binding of E 
      beta G-PZP3 alpha to proacrosin-acrosin may be involved in the binding of the ZP 
      to the acrosome of partially acrosome-reacted spermatozoa.
FAU - Yonezawa, N
AU  - Yonezawa N
AD  - Department of Chemistry, Faculty of Science, Chiba University, Japan.
FAU - Hatanaka, Y
AU  - Hatanaka Y
FAU - Takeyama, H
AU  - Takeyama H
FAU - Nakano, M
AU  - Nakano M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Reprod Fertil
JT  - Journal of reproduction and fertility
JID - 0376367
RN  - 0 (Egg Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Zona Pellucida Glycoproteins)
SB  - IM
MH  - Acrosome/*metabolism
MH  - Animals
MH  - Binding Sites
MH  - Blotting, Western
MH  - Egg Proteins/isolation & purification/*metabolism
MH  - Female
MH  - Histocytochemistry
MH  - Male
MH  - Membrane Glycoproteins/isolation & purification/*metabolism
MH  - Receptors, Cell Surface/*metabolism
MH  - Sperm-Ovum Interactions
MH  - Swine/*metabolism
MH  - Zona Pellucida/*metabolism
MH  - Zona Pellucida Glycoproteins
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1530/jrf.0.1030001 [doi]
PST - ppublish
SO  - J Reprod Fertil. 1995 Jan;103(1):1-8. doi: 10.1530/jrf.0.1030001.