PMID- 7708706
OWN - NLM
STAT- MEDLINE
DCOM- 19950511
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 92
IP  - 7
DP  - 1995 Mar 28
TI  - Two forms of acetylcholine receptor gamma subunit in mouse muscle.
PG  - 2686-90
AB  - Nicotinic acetylcholine receptors (nAcChoRs) of skeletal muscle are heterosubunit 
      ligand-gated channels that mediate signal transmission from motor nerves to 
      muscle. While cloning murine nAcChoR subunits, to gain an insight into the 
      receptor diversity across species, we detected two forms of gamma subunits in the 
      myogenic C2C12 cell line. Both forms are functional when expressed in Xenopus 
      oocytes. One gamma subunit [long gamma (gamma 1)] was almost identical to that 
      previously cloned in the murine BC3H-1 tumor cell line. The second form of gamma 
      subunit [short gamma (gamma s)] lacked 156 bp (52 amino acids) in the 
      extracellular N terminus, adjoining the hydrophobic segment M1, which corresponds 
      to the fifth exon of the gamma-subunit gene. The two forms of gamma subunit 
      coexist during myogenesis in vitro and in 17-day embryonic and denervated adult 
      muscle fibers in vivo. However, the gamma s variant was the only form of gamma 
      subunit in newborn muscle. In dissociated muscle fibers of newborn mice, 
      AcCho-evoked channel openings were more prolonged when compared with C2C12 
      myotubes or denervated adult muscle fibers. The gamma s subunit may, thus, 
      contribute to the structural and functional diversity of nAcChoRs in muscle 
      cells.
FAU - Mileo, A M
AU  - Mileo AM
AD  - Laboratorio di Biofisica, Centro Ricerca Sperimentale, IRE, Rome, Italy.
FAU - Monaco, L
AU  - Monaco L
FAU - Palma, E
AU  - Palma E
FAU - Grassi, F
AU  - Grassi F
FAU - Miledi, R
AU  - Miledi R
FAU - Eusebi, F
AU  - Eusebi F
LA  - eng
SI  - GENBANK/S77465
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Primers)
RN  - 0 (Macromolecular Substances)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Muscarinic)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA Primers
MH  - Embryo, Mammalian
MH  - Embryo, Nonmammalian
MH  - Exons
MH  - Female
MH  - Gene Expression
MH  - Macromolecular Substances
MH  - Membrane Potentials/drug effects
MH  - Mice
MH  - Molecular Sequence Data
MH  - Muscle, Skeletal/cytology/drug effects/*metabolism
MH  - Oocytes/drug effects/physiology
MH  - Patch-Clamp Techniques
MH  - Polymerase Chain Reaction
MH  - Protein Conformation
MH  - RNA, Messenger/isolation & purification/metabolism
MH  - Receptors, Muscarinic/*biosynthesis/chemistry
MH  - Transcription, Genetic
MH  - Xenopus laevis
PMC - PMC42283
EDAT- 1995/03/28 00:00
MHDA- 1995/03/28 00:01
PMCR- 1995/09/28
CRDT- 1995/03/28 00:00
PHST- 1995/03/28 00:00 [pubmed]
PHST- 1995/03/28 00:01 [medline]
PHST- 1995/03/28 00:00 [entrez]
PHST- 1995/09/28 00:00 [pmc-release]
AID - 10.1073/pnas.92.7.2686 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2686-90. doi: 10.1073/pnas.92.7.2686.