PMID- 7708907
OWN - NLM
STAT- MEDLINE
DCOM- 19950510
LR  - 20191023
IS  - 0301-634X (Print)
IS  - 0301-634X (Linking)
VI  - 33
IP  - 4
DP  - 1994
TI  - Modification of in vitro mouse embryogenesis by X-rays and fluorochromes.
PG  - 341-51
AB  - In view of planned studies using single particle irradiation at the Institute for 
      Medical Radiobiology (IMR), confocal microscopy will become an important tool to 
      visualise subtle changes in embryo morphology. Therefore, the influence of X-rays 
      and that of three different fluorochromes on the in vitro development of murine 
      2-cell stage embryos was investigated. Embryos of B6C3F1 mice were cultured at 32 
      h post-conception (pc) and treated with X-rays, acridine orange (AO), ethidium 
      bromide (EB) or propidium iodide (PI), respectively, in various doses (0.0-3.0 
      Gy) or concentrations (AO: 0.05-2.00 micrograms/ml; EB and PI: 0.50-10.00 
      micrograms/ml). Additional experiments using combinations of AO and irradiation 
      were performed. The embryos were cultivated for a total of 7 days and checked for 
      their vital status by morphological endpoints such as the percentage of embryos 
      that reached the blastocyst stage and the hatching rate. After treatment of the 
      2-cell embryos with AO, EB and PI, the dose-effect curves with the endpoint 
      'hatching rate' showed a 23-fold reduction of the ED50 for AO (0.23 microgram/ml) 
      compared with EB (5.30 micrograms/ml). Despite the higher toxicity, AO had much 
      better staining qualities in subtoxic concentrations than EB. PI showed no 
      toxicity in these experiments and was used as an inverse control for embryo 
      vitality. No synergistic modification of the radiogenic effects could be seen in 
      combination experiments (AO+X-rays).
FAU - Kohler, M
AU  - Kohler M
AD  - Institute for Medical Radiobiology, University of Zurich, Switzerland.
FAU - Kundig, A
AU  - Kundig A
FAU - Reist, H W
AU  - Reist HW
FAU - Michel, C
AU  - Michel C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Radiat Environ Biophys
JT  - Radiation and environmental biophysics
JID - 0415677
RN  - 0 (Fluorescent Dyes)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/pharmacology
MH  - Animals
MH  - Dose-Response Relationship, Radiation
MH  - Embryo, Mammalian/drug effects/physiology/*radiation effects
MH  - Female
MH  - Fluorescent Dyes/*pharmacology
MH  - Male
MH  - Mice
MH  - Microscopy, Confocal
MH  - Organ Culture Techniques
MH  - X-Rays
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1007/BF01210455 [doi]
PST - ppublish
SO  - Radiat Environ Biophys. 1994;33(4):341-51. doi: 10.1007/BF01210455.