PMID- 7709415
OWN - NLM
STAT- MEDLINE
DCOM- 19950509
LR  - 20190727
IS  - 0039-2499 (Print)
IS  - 0039-2499 (Linking)
VI  - 26
IP  - 4
DP  - 1995 Apr
TI  - Monocyte chemoattractant protein-1 messenger RNA expression in rat ischemic 
      cortex.
PG  - 661-5; discussion 665-6
AB  - BACKGROUND AND PURPOSE: Previously we demonstrated that focal cerebral ischemia 
      results in an increased expression of several cytokines/chemokines that precede 
      the infiltration of leukocytes into the ischemic cortex after focal stroke 
      induced by occlusion of the middle cerebral artery (MCAO). Monocyte 
      chemoattractant protein-1 (MCP-1) is a potent chemoattractant specific for 
      monocytes. The aim of the present study was to examine whether MCP-1 messenger 
      RNA (mRNA) is expressed in ischemic brain tissue after MCAO. METHODS: The 
      expression of MCP-1 mRNA in the ischemic cortex was first identified by means of 
      a sensitive reverse transcription and polymerase chain reaction technique. The 
      time course of expression of MCP-1 mRNA in the ischemic and nonischemic cerebral 
      cortex after both permanent MCAO and temporary MCAO (160 minutes) with 
      reperfusion was then examined by means of Northern blot analysis. RESULTS: Almost 
      no expression of MCP-1 mRNA was found in the sham-operated or nonischemic 
      (contralateral) cortex. A significant increase in MCP-1 mRNA expression in the 
      ischemic cortex was observed after either permanent or temporary MCAO. MCP-1 mRNA 
      was elevated at 6 hours (4.4-fold increase over sham; n = 4), reached its highest 
      expression from 12 hours to 2 days (22.7-fold at the peak level; P < .01), and 
      remained elevated up to 5 days (5.6-fold; P < .01) after permanent MCAO. The 
      profile of MCP-1 mRNA expression in the ischemic cortex after MCAO with 
      reperfusion was similar to that of permanent MCAO except that MCP-1 mRNA was 
      increased earlier (ie, 12.5-fold increase at 3 hours; n = 4; P < .01). Also, 
      MCP-1 mRNA expression in the ischemic cortex after permanent MCAO was 
      significantly greater in hypertensive rats than in two normotensive rats (n = 4; 
      P < .05). CONCLUSIONS: The demonstration of induced MCP-1 mRNA expression early 
      after focal ischemia suggests that MCP-1 may represent a locally expressed 
      monocyte chemoattractant that plays an important role in monocyte infiltration 
      into ischemic tissue and therefore may contribute to the tissue injury in 
      ischemic stroke. Further studies must concentrate on identifying the induced 
      expression of MCP-1 and its cellular localization in the ischemic brain when the 
      appropriate antibodies become available.
FAU - Wang, X
AU  - Wang X
AD  - Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, 
      King of Prussia, PA 19406, USA.
FAU - Yue, T L
AU  - Yue TL
FAU - Barone, F C
AU  - Barone FC
FAU - Feuerstein, G Z
AU  - Feuerstein GZ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Stroke
JT  - Stroke
JID - 0235266
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Brain Ischemia/*metabolism
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*biosynthesis
MH  - DNA, Complementary
MH  - Male
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Inbred SHR
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 10.1161/01.str.26.4.661 [doi]
PST - ppublish
SO  - Stroke. 1995 Apr;26(4):661-5; discussion 665-6. doi: 10.1161/01.str.26.4.661.