PMID- 7713509
OWN - NLM
STAT- MEDLINE
DCOM- 19950518
LR  - 20190816
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 24
IP  - 3
DP  - 1994 Dec
TI  - A novel family of cathepsin L-like (CTSLL) sequences on human chromosome 10q and 
      related transcripts.
PG  - 568-76
AB  - We have isolated a human genomic DNA cosmid clone while screening for the 
      cathepsin L gene that, when sequenced, revealed close similarity with but 
      significant differences from cDNA sequences that have been reported for cathepsin 
      L (CTSL). The clone bears a novel sequence that shows 88% identity to the coding 
      regions of the cathepsin L gene and a similar exon arrangement. We have called 
      this sequence the "human cathepsin L-like gene 1" (CTSLL1). Translating putative 
      exon sequences reveals a single premature stop codon; therefore no functional 
      products are likely to arise from this gene. Fluorescence in situ hybridization 
      (FISH) studies mapped the clone to chromosome 10q. Somatic cell hybrid mapping 
      confirmed the location of CTSLL1 to human chromosome 10 distinct from the 
      cathepsin L locus (CTSL) on chromosome 9. Furthermore, the FISH mapping studies 
      show that a family of at least three related sequences exists on chromosome 10q, 
      similar to the pattern of duplicated glutamate dehydrogenase (GLUD) gene loci 
      reported on 10q. Using PCR and sequencing with genomic DNA samples, we have 
      identified two additional novel related sequences (CTSLL2 and CTSLL3), and by PCR 
      analysis of cDNA samples we have identified corresponding transcripts. Comparison 
      of changes between our CTSLL1 sequence and the cathepsin L gene at mutation 
      insensitive sites suggests that the two sequences arose from a duplication event 
      40-50 million years ago, and therefore at the time of divergence of early 
      primates.
FAU - Bryce, S D
AU  - Bryce SD
AD  - Cancer Research Unit, Medical School, Newcastle University, Newcastle upon Tyne, 
      United Kingdom.
FAU - Lindsay, S
AU  - Lindsay S
FAU - Gladstone, A J
AU  - Gladstone AJ
FAU - Braithwaite, K
AU  - Braithwaite K
FAU - Chapman, C
AU  - Chapman C
FAU - Spurr, N K
AU  - Spurr NK
FAU - Lunec, J
AU  - Lunec J
LA  - eng
SI  - GENBANK/L07772
SI  - GENBANK/L25628
SI  - GENBANK/L25629
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Complementary)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.15 (CTSL protein, human)
RN  - EC 3.4.22.15 (Cathepsin L)
SB  - IM
GS  - CTSLL
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cathepsin L
MH  - Cathepsins/*genetics
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 10
MH  - Cloning, Molecular
MH  - Consensus Sequence
MH  - Cosmids
MH  - Cricetinae
MH  - Cysteine Endopeptidases
MH  - DNA Primers/genetics
MH  - DNA Probes/genetics
MH  - DNA, Complementary/genetics
MH  - *Endopeptidases
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Transcription, Genetic
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - S0888-7543(84)71667-3 [pii]
AID - 10.1006/geno.1994.1667 [doi]
PST - ppublish
SO  - Genomics. 1994 Dec;24(3):568-76. doi: 10.1006/geno.1994.1667.