PMID- 7713755
OWN - NLM
STAT- MEDLINE
DCOM- 19950518
LR  - 20190904
IS  - 0018-2214 (Print)
IS  - 0018-2214 (Linking)
VI  - 27
IP  - 1
DP  - 1995 Jan
TI  - CCD microscopy and image analysis of cells and chromosomes stained by 
      fluorescence in situ hybridization.
PG  - 4-14
AB  - This paper reviews methods and applications of CCD microscopy for analysing cells 
      and chromosomes subjected to fluorescence in situ hybridization (FISH). The 
      current status of indirect and direct FISH staining methods with respect to probe 
      labelling, detection sensitivity, multiplicity and DNA resolution is summarized. 
      Microscope hardware, including special multi-band pass filters and CCD cameras 
      required for FISH analysis, is described. Then follows a detailed discussion of 
      current and emerging applications such as the automated enumeration of 
      chromosomal abnormalities (counting of dots in interphase cells), comparative 
      genomic hybridization, automated evaluation of radiation-induced chromosomal 
      translocations, and high-resolution DNA mapping on highly extended chromatin. 
      Finally, the limitations of the present methodology and future prospects are 
      discussed.
FAU - Tanke, H J
AU  - Tanke HJ
AD  - Department of Cytochemistry and Cytometry, University of Leiden, The Netherlands.
FAU - Florijn, R J
AU  - Florijn RJ
FAU - Wiegant, J
AU  - Wiegant J
FAU - Raap, A K
AU  - Raap AK
FAU - Vrolijk, J
AU  - Vrolijk J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Netherlands
TA  - Histochem J
JT  - The Histochemical journal
JID - 0163161
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes/ultrastructure
MH  - Humans
MH  - Image Processing, Computer-Assisted/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Microscopy, Fluorescence/instrumentation/*methods
MH  - Photomicrography
RF  - 44
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1007/BF00164167 [doi]
PST - ppublish
SO  - Histochem J. 1995 Jan;27(1):4-14. doi: 10.1007/BF00164167.