PMID- 7714100
OWN - NLM
STAT- MEDLINE
DCOM- 19950512
LR  - 20071114
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 80
IP  - 4
DP  - 1995 Apr
TI  - Human uterine decidual macrophages express renin.
PG  - 1273-7
AB  - Human uterine decidual tissue contains many cell types, including stromal cells, 
      fibroblasts, and macrophages. Earlier studies have shown that decidualized 
      uterine stromal cells express renin, primarily in the form of prorenin. However, 
      the possibility that decidual macrophages, which comprise about 30% of the cells 
      in term decidua, also express renin has not been investigated. To determine 
      whether macrophages express renin, macrophages were isolated from enzymatically 
      dispersed term decidual cells using immunomagnetic beads coupled to antibodies to 
      human leukocyte antigen (HLA)-DR, an antigen present on macrophages, but not 
      other decidual cells. The isolated cells were 92.1% CD14 positive and contained 
      the messenger ribonucleic acids (mRNA) for the interleukin-2 type alpha receptor, 
      but not for PRL, a specific marker of decidualized stromal cells. 
      Immunocytochemical studies of the macrophage-enriched fraction demonstrated that 
      the macrophages contained renin, and reverse transcription-polymerase chain 
      reaction analysis with primers specific for renin indicated that the fraction 
      also contained renin mRNA. Renin was detected in the conditioned medium of 
      cultures of the macrophage-enriched preparations, greater than 90% of which was 
      in the form of prorenin. As anticipated, renin and renin mRNA were also detected 
      in the HLA-DR negative cells, more than 80% of which stained with specific 
      antiserum to PRL. Peripheral mononuclear cells also expressed renin mRNA, as 
      determined by reverse transcription-polymerase chain reaction analysis. These 
      results demonstrate that human decidual macrophages express renin and indicate 
      that renin is expressed by several cell types in decidual tissue.
FAU - Jikihara, H
AU  - Jikihara H
AD  - Division of Endocrinology, Children's Hospital Medical Center, Cincinnati, Ohio 
      45229, USA.
FAU - Poisner, A M
AU  - Poisner AM
FAU - Hirsch, R
AU  - Hirsch R
FAU - Handwerger, S
AU  - Handwerger S
LA  - eng
GR  - HD-15201/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Antibodies)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.23.15 (Renin)
SB  - IM
MH  - Antibodies/administration & dosage
MH  - Decidua/cytology/*metabolism
MH  - Female
MH  - HLA-DR Antigens/immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunologic Techniques
MH  - Macrophages/*metabolism
MH  - Magnetics
MH  - Microspheres
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/metabolism
MH  - Renin/genetics/*metabolism
MH  - Transcription, Genetic
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 10.1210/jcem.80.4.7714100 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1995 Apr;80(4):1273-7. doi: 10.1210/jcem.80.4.7714100.