PMID- 7714166
OWN - NLM
STAT- MEDLINE
DCOM- 19950517
LR  - 20190512
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 9
IP  - 12
DP  - 1994 Dec
TI  - Tumour necrosis factor alpha inhibits in-vitro decidualization of human 
      endometrial stromal cells.
PG  - 2411-7
AB  - Interleukin-1 (IL-1) has been reported previously to inhibit the in-vitro 
      decidualization of human endometrial stromal cells as assessed by 
      progesterone-induced prolactin production and morphological transformation. In 
      this study we examined whether other cytokines, such as tumour necrosis 
      factor-alpha (TNF alpha), interferon-beta (IFN beta), IFN gamma or 
      granulocyte-macrophage colony-stimulating factor (GM-CSF), could affect the 
      decidualization of human endometrial stromal cells in vitro. Of these cytokines, 
      TNF alpha significantly suppressed prolactin production in a dose-dependent 
      manner, with no apparent effect on cell number. The morphological transformation 
      of endometrial stromal cells was also inhibited by TNF alpha. TNF alpha and IL-1 
      significantly suppressed cAMP-stimulated prolactin production by endometrial 
      stromal cells. Neither the progesterone concentration in the supernatant of the 
      endometrial stromal cell culture system nor intracellular calcium concentration 
      of the endometrial stromal cells were affected by the addition of TNF alpha or 
      IL-1. These results indicated that TNF alpha and IL-1 suppress both 
      progesterone-induced and cAMP-mediated prolactin production in endometrial 
      stromal cells, and that this inhibition was not attributable to direct effects on 
      progesterone metabolism or related to Ca(2+)-mediated signal transduction. These 
      experiments suggested that a local increase of TNF alpha and IL-1 under certain 
      pathological conditions in vivo may disturb blastocyst implantation and/or the 
      maintenance of pregnancy by inhibiting the decidualization of endometrial stromal 
      cells.
FAU - Inoue, T
AU  - Inoue T
AD  - Department of Gynaecology and Obstetrics, Faculty of Medicine, Kyoto University, 
      Japan.
FAU - Kanzaki, H
AU  - Kanzaki H
FAU - Iwai, M
AU  - Iwai M
FAU - Imai, K
AU  - Imai K
FAU - Narukawa, S
AU  - Narukawa S
FAU - Higuchi, T
AU  - Higuchi T
FAU - Katsuragawa, H
AU  - Katsuragawa H
FAU - Mori, T
AU  - Mori T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 9002-62-4 (Prolactin)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Cells, Cultured
MH  - Cyclic AMP/pharmacology
MH  - Decidua/cytology/*drug effects/metabolism
MH  - Depression, Chemical
MH  - Female
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Progesterone/*antagonists & inhibitors/metabolism
MH  - Prolactin/biosynthesis
MH  - Stromal Cells/drug effects/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a138460 [doi]
PST - ppublish
SO  - Hum Reprod. 1994 Dec;9(12):2411-7. doi: 10.1093/oxfordjournals.humrep.a138460.