PMID- 7714828
OWN - NLM
STAT- MEDLINE
DCOM- 19950518
LR  - 20190512
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 482 ( Pt 2)
IP  - Pt 2
DP  - 1995 Jan 15
TI  - Effects of neurotensin on rat supraoptic nucleus neurones in vitro.
PG  - 373-81
AB  - 1. The electrophysiological actions of neurotensin on magnocellular 
      neurosecretory cells (MNCs) were examined during intracellular recording from 
      seventy-three supraoptic nucleus neurones in superfused explants of rat 
      hypothalamus. 2. Application of neurotensin tridecapeptide (NT(1-13); 1 nM to 3 
      microM) caused a membrane depolarization and reversibly attenuated the 
      after-hyperpolarization (AHP) which followed current-evoked spike trains. This 
      effect was accompanied by increased firing frequency during depolarizing current 
      pulses evoked from a fixed potential. 3. The effects of neurotensin could be 
      mimicked by the C-terminal fragment, NT(8-13), but not by the N-terminal 
      fragment, NT(1-8). 4. Depolarizing responses to NT(1-13) or NT(8-13), retained 
      during K+ channel blockade with internal Cs+, were accompanied by increased 
      membrane conductance. Current- and voltage-clamp analyses revealed that 
      neurotensin-evoked depolarizations result partly from the activation of a 
      non-selective cationic conductance reversing near -34 mV. 5. Depolarizing 
      responses to neurotensin were retained in the presence of TTX or in Ca(2+)-free 
      solutions, indicating the involvement of receptors located on the plasma membrane 
      of MNCs themselves. 6. Through these effects endogenously released neurotensin 
      may modulate excitability, activity patterns and secretion from the 
      hypothalamo-neurohypophysial axis.
FAU - Kirkpatrick, K
AU  - Kirkpatrick K
AD  - Centre for Research in Neuroscience, Montreal General Hospital, PQ, Canada.
FAU - Bourque, C W
AU  - Bourque CW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Potassium Channels)
RN  - 0 (Receptors, Neurotensin)
RN  - 39379-15-2 (Neurotensin)
SB  - IM
MH  - Animals
MH  - Electrophysiology
MH  - Hypothalamo-Hypophyseal System/*physiology
MH  - Male
MH  - Neurotensin/*physiology
MH  - Potassium Channels/physiology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Receptors, Neurotensin/physiology
MH  - Supraoptic Nucleus/*cytology/*drug effects
PMC - PMC1157735
EDAT- 1995/01/15 00:00
MHDA- 1995/01/15 00:01
PMCR- 1995/01/15
CRDT- 1995/01/15 00:00
PHST- 1995/01/15 00:00 [pubmed]
PHST- 1995/01/15 00:01 [medline]
PHST- 1995/01/15 00:00 [entrez]
PHST- 1995/01/15 00:00 [pmc-release]
AID - 10.1113/jphysiol.1995.sp020524 [doi]
PST - ppublish
SO  - J Physiol. 1995 Jan 15;482 ( Pt 2)(Pt 2):373-81. doi: 
      10.1113/jphysiol.1995.sp020524.