PMID- 77169
OWN - NLM
STAT- MEDLINE
DCOM- 19780715
LR  - 20191027
IS  - 0340-1057 (Print)
IS  - 0340-1057 (Linking)
VI  - 4
IP  - 2
DP  - 1978 Apr 13
TI  - Fluorescence polarization of stretched polytene chromosomes stained with acridine 
      orange.
PG  - 97-110
AB  - The molecules of the fluorescent dye acridine orange (AO) bind to DNA in such a 
      way that the absorption and emission dipoles lie on a plane perpendicular to the 
      DNA axis. For this reason, definite fluorescence polarization should correspond 
      to each mode of spatial DNA packing. A chromosome, considered as an axially 
      symmetrical ensemble of DNA, was characterized by two experimental parameters, P 
      parallel and P perpendicular, i.e., by polarizations of fluorescence excited by 
      light polarized parallel and perpendicular to the symmetry axis. In view of the 
      sequential order in the packing levels of DNA fiber in a chromosome, it was 
      suggested that, under mechanical stretching, the highest level is disrupted 
      first, then the others, in the order of their sequence. Isolated chromosomes of 
      Chironomus thummi were stained with AO and stretched with needles of a 
      micromanipulator. From the changes of P parallel and P perpendicular measured 
      during stretching it was concluded the polytene chromosome bands have three, at 
      least, DNA packing levels, tentatively described as 100 A fiber, 250 A coil and 
      chromomere.
FAU - Gruzdev, A D
AU  - Gruzdev AD
FAU - Kishchenko, G P
AU  - Kishchenko GP
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Biophys Struct Mech
JT  - Biophysics of structure and mechanism
JID - 7502020
RN  - 0 (Acridines)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Acridines
MH  - Animals
MH  - Chromosomes/*analysis
MH  - DNA/*analysis
MH  - Lepidoptera
MH  - Mathematics
MH  - Spectrometry, Fluorescence
MH  - Staining and Labeling
EDAT- 1978/04/13 00:00
MHDA- 1978/04/13 00:01
CRDT- 1978/04/13 00:00
PHST- 1978/04/13 00:00 [pubmed]
PHST- 1978/04/13 00:01 [medline]
PHST- 1978/04/13 00:00 [entrez]
AID - 10.1007/BF00539224 [doi]
PST - ppublish
SO  - Biophys Struct Mech. 1978 Apr 13;4(2):97-110. doi: 10.1007/BF00539224.