PMID- 7718884
OWN - NLM
STAT- MEDLINE
DCOM- 19950525
LR  - 20220224
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 85
IP  - 8
DP  - 1995 Apr 15
TI  - Detection of myc translocations in lymphoma cells by fluorescence in situ 
      hybridization with yeast artificial chromosomes.
PG  - 2132-8
AB  - Translocations involving chromosome 8 at band q24 and one of the Ig loci on 
      chromosomes 14q32, 22q11, and 2p11 are the hallmark of Burkitt's lymphoma (BL). 
      It has been previously observed that the exact localization of the breakpoints at 
      chromosome 8q24 can vary significantly from patient to patient, scattering over a 
      distance of more than 300 kb upstream of c-myc and about 300 kb downstream of 
      c-myc. To generate probes for fluorescence in situ hybridization (FISH) that 
      detect most c-myc translocations, we screened a yeast artificial chromosome (YAC) 
      library from normal human lymphocytes by colony hybridization, using three 
      markers surrounding the c-myc gene as probes. We obtained 10 YAC clones ranging 
      in size between 500 and 200 kb. Two nonchimeric clones were used for FISH on 
      several BL cell lines and patient samples with different breakpoints at 8q24. Our 
      results show that the YAC clones detected translocations scattered along 
      approximately 200 kb in both metaphase chromosomes and interphase nuclei. The 
      sensitivity, rapidity, and feasibility in nondividing cells render FISH an 
      important diagnostic tool. Furthermore, the use of large DNA fragments such as 
      YACs greatly simplifies the detection of translocations with widely scattered 
      breakpoints such as these seen in BL.
FAU - Veronese, M L
AU  - Veronese ML
AD  - Jefferson Cancer Institute, Jefferson Medical College, Philadelphia, PA 19107, 
      USA.
FAU - Ohta, M
AU  - Ohta M
FAU - Finan, J
AU  - Finan J
FAU - Nowell, P C
AU  - Nowell PC
FAU - Croce, C M
AU  - Croce CM
LA  - eng
GR  - CA 39860/CA/NCI NIH HHS/United States
GR  - CA 42232/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA, Neoplasm)
SB  - IM
GS  - c-myc
GS  - myc
MH  - Adult
MH  - Burkitt Lymphoma/*genetics/pathology
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes, Artificial, Yeast/*genetics
MH  - Chromosomes, Human, Pair 14/*ultrastructure
MH  - Chromosomes, Human, Pair 22/*ultrastructure
MH  - Chromosomes, Human, Pair 8/*ultrastructure
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Genes, Immunoglobulin
MH  - *Genes, myc
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphoma, Large-Cell, Immunoblastic/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1995/04/15 00:00
MHDA- 1995/04/15 00:01
CRDT- 1995/04/15 00:00
PHST- 1995/04/15 00:00 [pubmed]
PHST- 1995/04/15 00:01 [medline]
PHST- 1995/04/15 00:00 [entrez]
AID - S0006-4971(20)76940-5 [pii]
PST - ppublish
SO  - Blood. 1995 Apr 15;85(8):2132-8.