PMID- 7720245
OWN - NLM
STAT- MEDLINE
DCOM- 19950522
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 41
IP  - 4
DP  - 1995 Apr
TI  - HLA class II genotyping: two assay systems compared.
PG  - 553-6
AB  - In the last few years, a variety of DNA-based human leukocyte antigen (HLA) 
      typing methods have emerged, revealing the extreme polymorphism of HLA genes. 
      This polymorphism makes it difficult for a clinical laboratory to establish the 
      best HLA typing strategy. In this study we have compared two techniques for 
      performing HLA-DRB typing: a commercial rapid assay based on the polymerase chain 
      reaction (PCR) followed by reverse dot-blot hybridization of the PCR products 
      (the Inno-LiPA assay), and a method based on PCR followed by restriction fragment 
      length polymorphism analysis. We found that both methods provide reliable results 
      with a high rate of concordance (97%) and that Inno-LiPA is convenient for 
      large-scale routine typing. However, if a high-resolution allelic typing is 
      required, each method lacks accuracy but using them in association improves the 
      accuracy of the results.
FAU - Thonnard, J
AU  - Thonnard J
AD  - Clinical Laboratory of Molecular Biology, Louvain University Medical School, 
      Cliniques Universitaires St-Luc, Brussels, Belgium.
FAU - Deldime, F
AU  - Deldime F
FAU - Heusterspreute, M
AU  - Heusterspreute M
FAU - Delepaut, B
AU  - Delepaut B
FAU - Hanon, F
AU  - Hanon F
FAU - De Bruyere, M
AU  - De Bruyere M
FAU - Philippe, M
AU  - Philippe M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Alleles
MH  - *Genotype
MH  - Histocompatibility Antigens Class II/*genetics
MH  - Humans
MH  - Nucleic Acid Hybridization
MH  - *Polymerase Chain Reaction/statistics & numerical data
MH  - *Polymorphism, Restriction Fragment Length
MH  - Sensitivity and Specificity
MH  - Temperature
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
PST - ppublish
SO  - Clin Chem. 1995 Apr;41(4):553-6.