PMID- 7720585
OWN - NLM
STAT- MEDLINE
DCOM- 19950524
LR  - 20220215
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 121
IP  - 3
DP  - 1995 Mar
TI  - A role for HGF/SF in neural induction and its expression in Hensen's node during 
      gastrulation.
PG  - 813-24
AB  - It was previously shown (Roberts, C., Platt, N., Streit, A., Schachner, M. and 
      Stern, C. D. (1991) Development 112, 959-970) that grafts of Hensen's node into 
      chick embryos enhanced and maintain expression of the L5 carbohydrate in 
      neighbouring epiblast cells, and that antibodies against L5 inhibit neural 
      induction by such a graft. We now show that L5 is initially widely expressed in 
      the epiblast, but as neural induction proceeds it gradually becomes confined to 
      and up-regulated in the early neural plate. L5 can therefore be considered as a 
      marker for cells that are competent to respond to neural induction. We also show 
      that Hepatocyte Growth Factor/Scatter Factor (HGF/SF) promotes the expression of 
      L5 by extraembryonic epiblast in collagen gels after overnight culture. Explants 
      cultured for several days in the presence of HGF/SF, as well as explants of 
      prospective neural plate, can differentiate into cells with neuronal morphology 
      expressing neuronal markers. To investigate whether HGF/SF is expressed in the 
      chick embryo at appropriate stages of development, we produced specific cDNA 
      probes and used them for in situ hybridization. We find that at the primitive 
      streak stage, HGF/SF is expressed specifically in Hensen's node. We therefore 
      propose that HGF/SF plays a role during the early steps of neural induction, 
      perhaps by inducing or maintaining the competence of the epiblast to respond to 
      neural inducing signals.
FAU - Streit, A
AU  - Streit A
AD  - University of Manchester, UK.
FAU - Stern, C D
AU  - Stern CD
FAU - Thery, C
AU  - Thery C
FAU - Ireland, G W
AU  - Ireland GW
FAU - Aparicio, S
AU  - Aparicio S
FAU - Sharpe, M J
AU  - Sharpe MJ
FAU - Gherardi, E
AU  - Gherardi E
LA  - eng
SI  - GENBANK/X80131
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Antisense Elements (Genetics))
RN  - 0 (Biomarkers)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Antisense Elements (Genetics)
MH  - Base Sequence
MH  - Biomarkers/analysis
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Cloning, Molecular
MH  - Embryonic Induction/drug effects/*physiology
MH  - Gastrula/*physiology/transplantation
MH  - Gene Expression
MH  - Hepatocyte Growth Factor/genetics/pharmacology/*physiology
MH  - In Situ Hybridization
MH  - Molecular Sequence Data
MH  - Nervous System/cytology/drug effects/*embryology
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.1242/dev.121.3.813 [doi]
PST - ppublish
SO  - Development. 1995 Mar;121(3):813-24. doi: 10.1242/dev.121.3.813.