PMID- 7720642
OWN - NLM
STAT- MEDLINE
DCOM- 19950523
LR  - 20061115
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 136
IP  - 5
DP  - 1995 May
TI  - Tumor-stroma interactions and stromal cell density regulate hepatocyte growth 
      factor protein levels: a role for transforming growth factor-beta activation.
PG  - 1945-53
AB  - Hepatocyte growth factor/scatter factor (HGF/SF), a fibroblast-derived mediator 
      of epithelial and endothelial growth and motility, is regulated by factors 
      present in media conditioned by breast tumor cell lines. Both inhibitory and 
      stimulatory effects were observed dependent on culture conditions. The present 
      work shows that breast tumor cell conditioned medium contains a latent HGF/SF 
      inhibitory activity, which can be activated by a variety of treatments known to 
      activate latent transforming growth factor-beta. Using blocking antibodies and 
      other criteria, we show that transforming growth factor-beta present in 
      epithelial cell conditioned medium is primarily responsible for mediating the 
      down-regulation of fibroblast HGF/SF. Epithelial cell conditioned medium also 
      contains a trypsin-sensitive and heat-stable stimulatory activity. Stromal cell 
      density but not proliferation rate markedly alters HGF/SF expression. These 
      results indicate that the expression of at least one epithelial morphogen, 
      HGF/SF, is interdependently regulated by mesenchymal condensation and by factors 
      released by neighboring epithelial and carcinoma cells.
FAU - Seslar, S
AU  - Seslar S
AD  - Department of Cell Biology, Georgetown University Medical Center, Washington, 
      D.C. 20007, USA.
FAU - Nakamura, T
AU  - Nakamura T
FAU - Byers, S
AU  - Byers S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Antibodies)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Transforming Growth Factor beta)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Antibodies/pharmacology
MH  - Breast Neoplasms
MH  - Cell Communication
MH  - Cell Division
MH  - Cell Line
MH  - Culture Media, Conditioned
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells
MH  - Epithelium/physiology
MH  - Fetus
MH  - Hepatocyte Growth Factor/analysis/*biosynthesis/pharmacology
MH  - Humans
MH  - Kinetics
MH  - Lung
MH  - Temperature
MH  - Transforming Growth Factor beta/immunology/*physiology
MH  - Trypsin/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - 10.1210/endo.136.5.7720642 [doi]
PST - ppublish
SO  - Endocrinology. 1995 May;136(5):1945-53. doi: 10.1210/endo.136.5.7720642.