PMID- 7725819
OWN - NLM
STAT- MEDLINE
DCOM- 19950525
LR  - 20061115
IS  - 0236-5383 (Print)
IS  - 0236-5383 (Linking)
VI  - 45
IP  - 2-4
DP  - 1994
TI  - Effects of estrogen and estrogen receptor messenger RNA levels in young and 
      middle-aged female rats: comparison of medial preoptic area and mediobasal 
      hypothalamus.
PG  - 223-31
AB  - We examined the effects of estrogen on estrogen receptor (ER) mRNA in the rat 
      hypothalamus during aging. Two-month-old (young) and 9-month-old (middle-aged) 
      rats were ovariectomized (OVX) and after 10 days given an injection of estradiol 
      benzoate (20 micrograms/rat). The young rats were sacrificed 4 h, 24 h and 72 h, 
      and the middle-aged rats 72 h after estrogen injection. Control rats received a 
      sesame oil injection. The medial preoptic area (MPO) and mediobasal hypothalamus 
      (MBH) were dissected and extracted to obtain total RNAs. The levels of ER mRNA 
      were evaluated by northern blotting. We found that, in the MBH, estrogen 
      treatment decreased ER mRNA in young but not in middle-aged rats. In addition, ER 
      mRNA levels in middle-aged OVX rats were lower than in young OVX rats. By 
      contrast, estrogen failed to decrease ER mRNA in the MPO of young OVX rats. The 
      ER mRNA levels in the MPO of middle-aged OVX rats were significantly higher than 
      in any other groups. The present study indicates that the estrogenic regulation 
      of ER mRNA is different between the MBH and the MPO, and impaired in the MBH, but 
      not in the MPO of middle-aged rats.
FAU - Funabashi, T
AU  - Funabashi T
AD  - Department of Physiology, Yokohama City University School of Medicine, Japan.
FAU - Kimura, F
AU  - Kimura F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Hungary
TA  - Acta Biol Hung
JT  - Acta biologica Hungarica
JID - 8404358
RN  - 0 (DNA Primers)
RN  - 0 (Estrogens)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern/methods
MH  - DNA Primers
MH  - Estrogens/*physiology
MH  - Female
MH  - Hypothalamus, Middle/*metabolism
MH  - Molecular Sequence Data
MH  - Ovariectomy
MH  - Preoptic Area/*metabolism
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Estrogen/genetics/*metabolism
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PST - ppublish
SO  - Acta Biol Hung. 1994;45(2-4):223-31.