PMID- 7730433
OWN - NLM
STAT- MEDLINE
DCOM- 19950530
LR  - 20200409
IS  - 0166-0934 (Print)
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 51
IP  - 1
DP  - 1995 Jan
TI  - Comparative study of a blocking enzyme-linked immunosorbent assay and the 
      immunoperoxidase monolayer assay for the detection of antibodies to the porcine 
      reproductive and respiratory syndrome virus in pigs.
PG  - 125-8
AB  - A blocking enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to 
      the porcine reproductive and respiratory syndrome virus (PRRSV) in pig sera was 
      developed and compared with the immunoperoxidase monolayer assay (IPMA), the most 
      widely used serological diagnostic test in Europe. The blocking ELISA was 
      specific and more sensitive than the IPMA when applied to field sera and to sera 
      which were collected early after an experimental infection with PRRSV. Problems 
      with high background activity as observed in IPMA or indirect ELISA were not 
      encountered.
FAU - Houben, S
AU  - Houben S
AD  - Laboratory of Virology, Faculty of Veterinary Medicine, University of Gent, 
      Merelbeke, Belgium.
FAU - Callebaut, P
AU  - Callebaut P
FAU - Pensaert, M B
AU  - Pensaert MB
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Arterivirus/*immunology
MH  - Arterivirus Infections/diagnosis/immunology/veterinary
MH  - Binding, Competitive
MH  - Enzyme-Linked Immunosorbent Assay/methods/statistics & numerical data/*veterinary
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Immunoenzyme Techniques/statistics & numerical data/*veterinary
MH  - Maternal-Fetal Exchange/immunology
MH  - Pregnancy
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/diagnosis/immunology/transmission
MH  - Virology/methods/statistics & numerical data
PMC - PMC7119743
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
PMCR- 2000/04/18
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PHST- 2000/04/18 00:00 [pmc-release]
AID - 0166093494001354 [pii]
AID - 0166-0934(94)00135-4 [pii]
AID - 10.1016/0166-0934(94)00135-4 [doi]
PST - ppublish
SO  - J Virol Methods. 1995 Jan;51(1):125-8. doi: 10.1016/0166-0934(94)00135-4.