PMID- 7736778
OWN - NLM
STAT- MEDLINE
DCOM- 19950606
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 70
IP  - 1-2
DP  - 1995
TI  - Precise ordering of 26 cosmid markers on chromosome region 3p23-->p21.3 by 
      two-color FISH on human prophase chromosomes and stretched DNAs.
PG  - 129-33
AB  - To construct a detailed cytogenetic map of human chromosome region 3p23-->p21.3, 
      we determined the order of 26 cosmid markers (cCI 3 series) previously localized 
      within this region by fluorescence in situ hybridization (FISH). Two-color 
      pairwise FISH analysis of prophase chromosomes provided the order of these 
      markers as follows: pter - 245 (D3S647) - 872 (D3S1018) - 818 (D3S996) - 905 
      (D3S1022) - 515 (D3S685) - 1195 (D3S1125) - 718 (D3S935) - 911 (D3S1025) - 878 
      (D3S1020) - 717 (D3S934) - 401 (D3S664) - [708 (D3S926)/524 (D3S686)] - 848 
      (D3S1011) - 771 (D3S966) - 917 (D3S1029) - 533 (D3S688) - 470 (D3S676) - 940 
      (D3S1037) - 785 (D3S974) - 810 (D3S988) - 9 (D3S643) - 382 (D3S660) - 769 
      (D3S965) - 792 (D3S978) - 604 (D3S705) - cen. The two-color signals of 524 
      (D3S686) and 708 (D3S926) were visualized as an overlapping pattern on prophase 
      chromosomes, and, further, the string signals also overlapped on stretched DNAs, 
      allowing us to determine their precise order as pter - D3S926 - D3S686 - cen. The 
      precise order of 26 DNA markers on 3p23-->p21.3 can provide useful information 
      for the positional cloning of tumor suppressor gene(s) and cancer breakpoint(s) 
      encompassed in this region.
FAU - Ariyama, T
AU  - Ariyama T
AD  - Department of Hygiene, Kyoto Prefectural University of Medicine, Japan.
FAU - Kimura, T
AU  - Kimura T
FAU - Yamakawa, K
AU  - Yamakawa K
FAU - Nakamura, Y
AU  - Nakamura Y
FAU - Abe, T
AU  - Abe T
FAU - Inazawa, J
AU  - Inazawa J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 3
MH  - Cosmids/*genetics
MH  - DNA/*genetics
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Prophase/genetics
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1159/000134077 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1995;70(1-2):129-33. doi: 10.1159/000134077.