PMID- 7738406
OWN - NLM
STAT- MEDLINE
DCOM- 19950608
LR  - 20190909
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 67
IP  - 4
DP  - 1995 Apr
TI  - Frequencies of complex chromosome exchange aberrations induced by 238Pu 
      alpha-particles and detected by fluorescence in situ hybridization using single 
      chromosome-specific probes.
PG  - 431-9
AB  - We undertook an analysis of chromosome-type exchange aberrations induced by 
      alpha-particles using fluorescence in situ hybridization (FISH) with whole 
      chromosome-specific probes for human chromosomes 1 or 4, together with a 
      pan-centromeric probe. Contact-inhibited primary human fibroblasts (in G1) were 
      irradiated with 0.41-1.00 Gy 238Pu alpha-particles and aberrations were analysed 
      at the next mitosis following a single chromosome paint. Exchange and aberration 
      painting patterns were classified according to Savage and Simpson (1994a). Of 
      exchange aberrations, 38-47% were found to be complex derived, i.e. resulting 
      from three or more breaks in two or more chromosomes, and the variation with dose 
      was minimal. The class of complex aberrations most frequently observed were 
      insertions, derived from a minimum of three breaks in two chromosomes. There was 
      also an elevated frequency of rings. The high level of complex aberrations 
      observed after alpha-particle irradiation indicates that, when chromosome domains 
      are traversed by high linear energy transfer alpha-particle tracks, there is an 
      enhanced probability of production of multiple localized double-strand breaks 
      leading to more complicated interactions.
FAU - Griffin, C S
AU  - Griffin CS
AD  - MRC Radiobiology Unit, Chilton, Didcot, UK.
FAU - Marsden, S J
AU  - Marsden SJ
FAU - Stevens, D L
AU  - Stevens DL
FAU - Simpson, P
AU  - Simpson P
FAU - Savage, J R
AU  - Savage JR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (DNA Probes)
RN  - 53023GN24M (Plutonium)
SB  - IM
MH  - *Alpha Particles
MH  - Cell Nucleus/radiation effects
MH  - Cell Survival/radiation effects
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1/radiation effects
MH  - Chromosomes, Human, Pair 4/radiation effects
MH  - *DNA Probes
MH  - Fibroblasts/*physiology/*radiation effects/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Plutonium/*pharmacology
MH  - Sensitivity and Specificity
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 6KMW44A7DXP0U6BK [pii]
AID - 10.1080/09553009514550491 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1995 Apr;67(4):431-9. doi: 10.1080/09553009514550491.