PMID- 7739991
OWN - NLM
STAT- MEDLINE
DCOM- 19950602
LR  - 20190904
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 15
IP  - 1
DP  - 1995 Jan
TI  - Rapid prenatal diagnosis of 14 cases of triploidy using fish with multiple 
      probes.
PG  - 1-5
AB  - Fluorescence in situ hybridization (FISH) of chromosome-specific probes to 
      interphase nuclei can rapidly identify aneuploidies in uncultured amniotic fluid 
      cells. Using DNA probe sets specific for chromosomes 13, 18, 21, X, and Y, we 
      have identified 14 fetuses where the hybridization pattern was consistent with a 
      triploid chromosome constitution. In each case, the identification of fetal 
      abnormalities by ultrasound examination initiated a request for rapid 
      determination of ploidy status via prenatal FISH analysis of uncultured 
      amniocytes. FISH produced a three-signal pattern for the three autosomes in 
      combination with signals indicating an XXX or XXY sex chromosome complement. This 
      hybridization pattern was interpreted to be consistent with triploidy. Results 
      were reported to the physician within 2 days of amniocentesis and subsequently 
      confirmed by cytogenetics. These cases demonstrate the utility of FISH for rapid 
      prenatal identification of triploidy, particularly when fetal abnormalities are 
      seen with ultrasonographic examination.
FAU - Gersen, S L
AU  - Gersen SL
AD  - Integrated Genetics, Framingham, Massachusetts, USA.
FAU - Carelli, M P
AU  - Carelli MP
FAU - Klinger, K W
AU  - Klinger KW
FAU - Ward, B E
AU  - Ward BE
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (DNA Probes)
SB  - IM
MH  - Amniocentesis
MH  - *Chromosome Aberrations
MH  - *DNA Probes
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Sex Chromosome Aberrations
MH  - Time Factors
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1002/pd.1970150102 [doi]
PST - ppublish
SO  - Prenat Diagn. 1995 Jan;15(1):1-5. doi: 10.1002/pd.1970150102.