PMID- 7740005
OWN - NLM
STAT- MEDLINE
DCOM- 19950602
LR  - 20190904
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 15
IP  - 1
DP  - 1995 Jan
TI  - Mid-trimester fetal sex determination from maternal peripheral blood by 
      fluorescence in situ hybridization without enrichment of fetal cells.
PG  - 78-81
AB  - To determine the fetal sex on 30 women who were 16-20 weeks pregnant, about 
      100,000 maternal blood nucleated cells were analysed by means of fluorescence in 
      situ hybridization (FISH) with a Y-chromosome-specific DNA probe. Cells with the 
      hybridization signal were detected in 12 of the 30 women. All the 12 mothers gave 
      birth to a male child. Of the other 18 women who had no Y-positive cells in the 
      peripheral blood, 14 gave birth to a female child and four gave birth to a male 
      child. These false-negative results probably occurred because the number of cells 
      examined was inadequate. The data obtained in this study suggest that fetal sex 
      determination using maternal peripheral blood with FISH is possible and that this 
      diagnostic method will be clinically useful when more cells are analysed.
FAU - Hamada, H
AU  - Hamada H
AD  - Department of Obstetrics and Gynecology, University of Tsukuba, Ibaraki, Japan.
FAU - Arinami, T
AU  - Arinami T
FAU - Sohda, S
AU  - Sohda S
FAU - Hamaguchi, H
AU  - Hamaguchi H
FAU - Kubo, T
AU  - Kubo T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (DNA Probes)
SB  - IM
MH  - Blood Cells
MH  - DNA Probes
MH  - False Negative Reactions
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pregnancy
MH  - Pregnancy Trimester, Second
MH  - *Prenatal Diagnosis
MH  - Sex Determination Analysis/*methods
MH  - Y Chromosome
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1002/pd.1970150117 [doi]
PST - ppublish
SO  - Prenat Diagn. 1995 Jan;15(1):78-81. doi: 10.1002/pd.1970150117.