PMID- 7740833
OWN - NLM
STAT- MEDLINE
DCOM- 19950607
LR  - 20150901
IS  - 0513-5796 (Print)
IS  - 0513-5796 (Linking)
VI  - 36
IP  - 1
DP  - 1995 Mar
TI  - Induction of ICAM-1 and HLA-DR expression by IFN-gamma in malignant melanoma cell 
      lines.
PG  - 15-25
AB  - Two human malignant melanoma cell lines, Malme-3M and SK-Mel-28, were analyzed 
      for their ability to induce the expression of intercellular adhesion molecule 1 
      (ICAM-1) and human leukocyte antigen (HLA)-DR molecules on their cell surfaces as 
      well as at the transcriptional level before and after treatment with interferon 
      (IFN)-gamma. Both cell lines demonstrated a high percentage (> 99%) of ICAM-1 
      expression regardless of IFN-gamma treatment. Before IFN-gamma treatment, 
      Malme-3M cells barely expressed HLA-DR molecules (< 2%) and SK-Mel-28 cells 
      demonstrated a relatively high percentage (> 50%) of HLA-DR expression. Both cell 
      lines displayed elevated levels of HLA-DR expression in a time dependent manner 
      after IFN-gamma treatment. However, these two cell lines have been shown to 
      respond differentially to IFN-gamma. The molecular mechanism underlying such a 
      differential behavior was investigated, and HLA-DR gene regulation was studied at 
      the transcriptional level. Treatment with IFN-gamma led to the steady-state mRNA 
      augmentation of the HLR-DR gene. The HLA-DRA mRNA augmentation was similar in 
      both cell lines, whereas in Malme-3M, IFN-gamma did not augment the rate of 
      transcription of the HLA-DRB gene as much as in SK-Mel-28. Data from this study 
      established the fact that the melanoma cell lines displayed a differential 
      susceptibility to IFN-gamma on the modulation of HLA-DR molecules, and this 
      modulation was transcriptionally regulated.
FAU - Kim, J D
AU  - Kim JD
AD  - Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
FAU - Lee, J L
AU  - Lee JL
FAU - Park, J H
AU  - Park JH
FAU - Lee, J M
AU  - Lee JM
FAU - Kim, Y H
AU  - Kim YH
FAU - Kim, S J
AU  - Kim SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - Yonsei Med J
JT  - Yonsei medical journal
JID - 0414003
RN  - 0 (HLA-DR Antigens)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Genes, MHC Class II
MH  - HLA-DR Antigens/*metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/*metabolism
MH  - Interferon-gamma/*pharmacology
MH  - Melanoma/*metabolism/pathology
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.3349/ymj.1995.36.1.15 [doi]
PST - ppublish
SO  - Yonsei Med J. 1995 Mar;36(1):15-25. doi: 10.3349/ymj.1995.36.1.15.