PMID- 7742547
OWN - NLM
STAT- MEDLINE
DCOM- 19950609
LR  - 20231213
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 85
IP  - 10
DP  - 1995 May 15
TI  - TEL gene is involved in myelodysplastic syndromes with either the typical 
      t(5;12)(q33;p13) translocation or its variant t(10;12)(q24;p13).
PG  - 2848-52
AB  - A t(5;12)(q33;p13) translocation is a recurrent chromosome abnormality in a 
      subgroup of myeloid malignancies with features of both myeloproliferative 
      disorders and myelodysplastic syndromes (MDSs). The molecular consequence of a 
      t(5;12) is a fusion between the platelet-derived growth factor receptor-B gene on 
      chromosome 5 and a novel ETS-like gene, TEL, on chromosome 12. We report on three 
      patients with a t(5;12)(q33;p13) diagnosed as chronic myelomonocytic leukemia, 
      and one case of a t(10;12)(q24;p13) in a progressive MDS, with eosinophilia and 
      monocytosis. Involvement of the TEL gene in these chromosome translocations was 
      investigated by fluorescence in situ hybridization (FISH) with cosmid probes 
      containing selectively the 5' end or 3' end of TEL. Hybridization of these 
      cosmids to the der(5)/der(10) or a der(12), respectively, demonstrated a 
      rearrangement of TEL in both translocations, showing that the t(10;12) is a 
      variant translocation of the t(5;12). Cloning of the fusion cDNA of one case of 
      t(5;12) showed that the breakpoint occurred at the RNA level at exactly the same 
      position as reported by Golub et al (Cell 77:307, 1994). In addition, the TEL 
      gene on chromosome 12 could be localized between two probes previously mapped to 
      12p13, namely PRB1 and D12S178, leading to a better definition of the position of 
      TEL in this chromosome region. Moreover, in the case involving chromosome 10, the 
      breakpoint occurred between cKTN206 and cKTN312/LYT-10 at 10q24. 
      Clinicohematological data in these studies as well as the restriction mapping of 
      chromosomal breakpoints strongly suggest that (1) common features in MDSs 
      involving the TEL gene are monocytosis and eosinophilia, (2) chromosomes other 
      than no. 5 may be involved and at least a t(10;12)(q24;p13) variant chromosome 
      translocation does exist in these MDSs, and (3) both standard and variant 12p/TEL 
      translocations may be identified by FISH with appropriate probes.
FAU - Wlodarska, I
AU  - Wlodarska I
AD  - Center for Human Genetics, University of Leuven, Belgium.
FAU - Mecucci, C
AU  - Mecucci C
FAU - Marynen, P
AU  - Marynen P
FAU - Guo, C
AU  - Guo C
FAU - Franckx, D
AU  - Franckx D
FAU - La Starza, R
AU  - La Starza R
FAU - Aventin, A
AU  - Aventin A
FAU - Bosly, A
AU  - Bosly A
FAU - Martelli, M F
AU  - Martelli MF
FAU - Cassiman, J J
AU  - Cassiman JJ
AU  - et al.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins c-ets)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.10.1 (Receptors, Platelet-Derived Growth Factor)
SB  - IM
GS  - PDGRFB
GS  - TEL
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - Chromosome Aberrations/genetics
MH  - Chromosome Disorders
MH  - Chromosomes, Human, Pair 10
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 5
MH  - DNA Primers/chemistry
MH  - DNA, Neoplasm/genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelomonocytic, Chronic/*genetics
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Myelodysplastic Syndromes/*genetics
MH  - Proto-Oncogene Proteins c-ets
MH  - RNA, Neoplasm/genetics
MH  - Receptors, Platelet-Derived Growth Factor/genetics
MH  - *Repressor Proteins
MH  - Transcription Factors/*genetics
MH  - Translocation, Genetic
MH  - ETS Translocation Variant 6 Protein
EDAT- 1995/05/15 00:00
MHDA- 1995/05/15 00:01
CRDT- 1995/05/15 00:00
PHST- 1995/05/15 00:00 [pubmed]
PHST- 1995/05/15 00:01 [medline]
PHST- 1995/05/15 00:00 [entrez]
AID - S0006-4971(20)76459-1 [pii]
PST - ppublish
SO  - Blood. 1995 May 15;85(10):2848-52.