PMID- 7743604
OWN - NLM
STAT- MEDLINE
DCOM- 19950613
LR  - 20061115
IS  - 0092-6213 (Print)
IS  - 0092-6213 (Linking)
VI  - 44
IP  - 2
DP  - 1994 Oct
TI  - Potentiation of the toxicity of tumor necrosis factor by tumors in mice.
PG  - 77-83
AB  - Tumor necrosis factor-alpha (TNF-alpha) was reported to be important in the 
      induction of septic shock. After i.v. injection of recombinant TNF-alpha 
      (rTNF-alpha), BALB/c mice bearing Meth A fibrosarcoma (Meth A), but not normal 
      mice, died of shock. Tumor cells are known to release many biological components. 
      In this study, we examined the role of the tumor in the toxicity of rTNF-alpha in 
      mice. Meth A cells maintained i.p. in mice were cultured for 24 hr in vitro. 
      Conditioned medium (CM) obtained from the Meth A cells was given i.v. to mice, 
      and 2 to 7 days later, i.v. injection of rTNF-alpha induced death in the animals. 
      rTNF-alpha treatment 4 days after Meth A CM gave the maximum effect. rTNF-alpha 
      did not induce death in mice treated with CM from spleen cells. However, after 
      the Meth A cells were passaged 2 or 3 times in in vitro culture, the CM did not 
      potentiate the toxicity of rTNF-alpha in mice. rTNF-alpha induced symptoms of 
      disseminated intravascular coagulation (DIC) on coagulation parameters in the 
      blood, and high plasma tissue factor (TF) activity in Meth A CM-treated mice and 
      Meth A tumor-bearing mice. These results suggest that factor(s) are released from 
      tumor cells activated by interaction with host cells, and injection of rTNF-alpha 
      and the factor(s) results in the induction of DIC syndrome leading to host death.
FAU - Nishigaki, F
AU  - Nishigaki F
AD  - Department of Pharmacology, Fujisawa Pharmaceutical Co., Ltd., Osaka, Japan.
FAU - Miyayasu, K
AU  - Miyayasu K
FAU - Tsujimoto, S
AU  - Tsujimoto S
FAU - Manda, T
AU  - Manda T
FAU - Shimomura, K
AU  - Shimomura K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Circ Shock
JT  - Circulatory shock
JID - 0414112
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9035-58-9 (Thromboplastin)
SB  - IM
MH  - Animals
MH  - Female
MH  - Injections, Intravenous
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neoplasms, Experimental/blood/*pathology
MH  - Thromboplastin/metabolism
MH  - Tumor Cells, Cultured/drug effects
MH  - Tumor Necrosis Factor-alpha/*toxicity
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
PST - ppublish
SO  - Circ Shock. 1994 Oct;44(2):77-83.