PMID- 7745546
OWN - NLM
STAT- MEDLINE
DCOM- 19950612
LR  - 20220331
IS  - 0904-2512 (Print)
IS  - 0904-2512 (Linking)
VI  - 24
IP  - 2
DP  - 1995 Feb
TI  - Identification and characterization of gelatinases/type IV collagenases in jaw 
      cysts.
PG  - 78-84
AB  - The molecular mechanisms of jaw cyst expansion probably involve interactions of 
      matrix metalloproteinases (MMPs) and the tissue inhibitors of MMPs (TIMPs). In 
      this study, molecular species of gelatinases present in neutral salt extracts of 
      cyst walls and cyst fluids were characterized by functional activity measurements 
      (type I gelatin and alpha-casein zymography) and immunologically 
      (Western-blotting). The effects of various protein thiol-group or cysteine-switch 
      reactants involved in the activation of collagenases were studied on cyst 
      gelatinases and a gelatinases purified from human gingival fibroblasts (72 kD 
      MMP-2), gingival keratinocytes (92 kD MMP-9) and polymorphonuclear neutrophilic 
      leukocytes (92 kD MMP-9). Western-blotting revealed the presence of both 92 kD 
      (MMP-9) and 72 kD (MMP-2) gelatinases in cyst wall extracts and cyst fluids. 
      Western-blot studies further suggested that jaw cyst gelatinases were only in 
      part complexed with and thus inhibited by TIMP-1 or TIMP-2, suggesting that both 
      MMP-9 and MMP-2 may participate in cyst expansion. MMP-2 was also partially 
      fragmented to a 68 kD form and additional lower molecular weight proteinases (< 
      60 kD) were detected by alpha-casein zymography and by Western-blotting, 
      suggesting proteolytic fragmentation. MMP-9 was at least partially activated by 
      all protein-thiol group reactants and rather resistant to oxidative inhibition by 
      hypochlorite (NaOCl); in contrast, MMP-2 was activated by APMA but not at all by 
      gold thioglucose (GTG) and was clearly inactivated by hypochlorite 
      (NaOCl).(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Teronen, O
AU  - Teronen O
AD  - Department of Oral and Maxillofacial Surgery, University of Helsinki, Finland.
FAU - Salo, T
AU  - Salo T
FAU - Konttinen, Y T
AU  - Konttinen YT
FAU - Rifkin, B
AU  - Rifkin B
FAU - Vernillo, A
AU  - Vernillo A
FAU - Ramamurthy, N S
AU  - Ramamurthy NS
FAU - Kjeldsen, L
AU  - Kjeldsen L
FAU - Borregaard, N
AU  - Borregaard N
FAU - Hietanen, J
AU  - Hietanen J
FAU - Sorsa, T
AU  - Sorsa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Proteins)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Gelatinases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Blotting, Western
MH  - Child
MH  - Collagenases/*metabolism
MH  - Extracellular Matrix Proteins/metabolism
MH  - Female
MH  - Gelatinases/*metabolism
MH  - Glycoproteins/metabolism
MH  - Histocytochemistry
MH  - Humans
MH  - Jaw Cysts/*enzymology
MH  - Male
MH  - Matrix Metalloproteinase 2
MH  - Matrix Metalloproteinase 9
MH  - Metalloendopeptidases/antagonists & inhibitors/metabolism
MH  - Middle Aged
MH  - Neutrophil Activation
MH  - Proteins/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2
MH  - Tissue Inhibitor of Metalloproteinases
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 10.1111/j.1600-0714.1995.tb01143.x [doi]
PST - ppublish
SO  - J Oral Pathol Med. 1995 Feb;24(2):78-84. doi: 10.1111/j.1600-0714.1995.tb01143.x.