PMID- 7745696
OWN - NLM
STAT- MEDLINE
DCOM- 19950613
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 69
IP  - 6
DP  - 1995 Jun
TI  - Identification and characterization of a cDNA derived from multiple splicing that 
      encodes envelope glycoprotein gp105 of human herpesvirus 6.
PG  - 3490-500
AB  - The glycoprotein complex gp82-gp105 is a major virion envelope glycoprotein 
      complex of human herpesvirus 6 variant A (HHV-6A) and consists of a number of 
      related polypeptides. Monoclonal antibodies (MAbs) 2D4, 2D6, and 13D6 against 
      this glycoprotein complex neutralized HHV-6A infectivity. We have previously 
      reported the isolation, mapping, and characterization of a portion of the viral 
      genomic DNA fragment encoding the gp82-gp105 complex and the identification of 
      the neutralizing epitope (B. Pfeiffer, Z. N. Berneman, F. Neipel, C. K. Chang, S. 
      Tirwatnapong, and B. Chandran, J. Virol. 67:4611-4620, 1993). This gene was 
      further characterized by the identification of a 2.3-kb genomic fragment and by 
      the identification of a 2.5-kb cDNA clone. The genomic sequence contains a short 
      open reading frame (ORF) encoding the epitope recognized by the MAbs. The 
      identified cDNA showed specificity for HHV-6 in Southern blot analysis with viral 
      DNA. In Northern (RNA) blot analysis with total RNA from HHV-6A(GS)-infected 
      cells, the cDNA insert specifically hybridized with several RNA species. 
      Restriction mapping analysis localized this cDNA to the HHV-6A(U1102) genomic 
      BamHI G fragment, at the right end of the unique long segment of the genome and 
      to the SalI L and SalI O fragments within the left and right terminal direct 
      repeat regions, respectively. In vitro transcription and translation of the cDNA 
      revealed a polypeptide of about 88.5 kDa which was glycosylated in the presence 
      of microsomal membranes to a polypeptide of approximately 104.2 kDa. Both 
      polypeptides were immunoprecipiated by MAb 2D6, verifying the identity of the 
      cDNA as encoding the gp105 in the gp82-gp105 complex. Sequence analysis of the 
      cDNA revealed a large ORF potentially encoding a 650-amino-acid protein with 11 
      potential N-linked glycosylation sites and 18 cysteine residues. A potential 
      membrane-spanning domain is located only near the amino terminus of the putative 
      protein, indicating that gp105 may be a class 2 glycoprotein. Comparison of the 
      cDNA nucleotide sequence with sequences from HHV-6A(U1102) genomic BamHI G and 
      SalI L fragments revealed that the gene encoding gp105 contains 12 exons, 
      spanning over 20 kb of the viral genome, with intron 1 spanning about 8 kb of 
      genomic DNA. The first exon of the cDNA mapped to the right and left terminal 
      direct repeats, while the other exons mapped within the unique long segment of 
      the genome.(ABSTRACT TRUNCATED AT 400 WORDS)
FAU - Pfeiffer, B
AU  - Pfeiffer B
AD  - Department of Microbiology, Molecular Genetics and Immunology, University of 
      Kansas Medical Center, Kansas City 66160, USA.
FAU - Thomson, B
AU  - Thomson B
FAU - Chandran, B
AU  - Chandran B
LA  - eng
SI  - GENBANK/U23466
SI  - GENBANK/U23467
SI  - GENBANK/U43400
GR  - AI 24224/AI/NIAID NIH HHS/United States
GR  - AI 30356/AI/NIAID NIH HHS/United States
GR  - AI 33502/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Viral)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 105, Human herpesvirus 6)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - DNA, Viral
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Membrane Glycoproteins/*genetics
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Protein Biosynthesis
MH  - *RNA Splicing
MH  - RNA, Messenger/genetics
MH  - Transcription, Genetic
MH  - Viral Envelope Proteins/*genetics
PMC - PMC189062
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
PMCR- 1995/06/01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
PHST- 1995/06/01 00:00 [pmc-release]
AID - 10.1128/JVI.69.6.3490-3500.1995 [doi]
PST - ppublish
SO  - J Virol. 1995 Jun;69(6):3490-500. doi: 10.1128/JVI.69.6.3490-3500.1995.