PMID- 7747417
OWN - NLM
STAT- MEDLINE
DCOM- 19950609
LR  - 20081121
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 208
IP  - 2
DP  - 1995 Apr 20
TI  - The retinoblastoma gene product negatively regulates transcriptional activation 
      mediated by the human cytomegalovirus IE2 protein.
PG  - 450-6
AB  - The IE2 gene product of human cytomegalovirus (HCMV) is one of a few viral 
      regulatory proteins expressed immediately upon infection of the host cell. It is 
      a potent transcriptional activator of many viral and cellular promoters. We found 
      that the retinoblastoma susceptibility gene product (Rb) dramatically suppressed 
      this IE2 transactivation of various promoters. However, unlike another tumor 
      suppressor protein, p53, Rb did not have any significant effect on basal levels 
      of transcription, suggesting that Rb specifically interacts with IE2 rather than 
      other cellular factors involved in the general transcription machinery. We found 
      by protein-affinity chromatography that Rb in nuclear extracts or produced by in 
      vitro translation directly bound to IE2. Our results suggest that Rb may regulate 
      the life cycle of HCMV, which is endemic in the human population. Furthermore, 
      these data may provide new insights into the slow rate of HCMV DNA replication in 
      cells and the possible involvement of HCMV in tumorigenesis.
FAU - Choi, K S
AU  - Choi KS
AD  - Institute for Molecular Biology and Genetics, College of Natural Sciences, Seoul 
      National University, Korea.
FAU - Kim, S J
AU  - Kim SJ
FAU - Kim, S
AU  - Kim S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Gene Products, tat)
RN  - 0 (IE2 protein, Cytomegalovirus)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Trans-Activators)
RN  - 0 (UL115 protein, Human herpesvirus 5)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (glycoprotein H, Cytomegalovirus)
RN  - 0 (glycoprotein H, Human cytomegalovirus)
RN  - 0 (glycoprotein O, cytomegalovirus)
SB  - IM
MH  - Cell Line
MH  - *Cytomegalovirus
MH  - Down-Regulation
MH  - Gene Products, tat/genetics
MH  - Humans
MH  - Immediate-Early Proteins/genetics/*metabolism
MH  - *Membrane Glycoproteins
MH  - Monocytes/cytology
MH  - Promoter Regions, Genetic/genetics
MH  - Recombinant Fusion Proteins/metabolism
MH  - Retinoblastoma Protein/genetics/*metabolism/physiology
MH  - Trans-Activators/genetics/*metabolism
MH  - Transcription, Genetic
MH  - Transcriptional Activation/*physiology
MH  - Transfection
MH  - *Viral Envelope Proteins
MH  - *Viral Proteins
EDAT- 1995/04/20 00:00
MHDA- 1995/04/20 00:01
CRDT- 1995/04/20 00:00
PHST- 1995/04/20 00:00 [pubmed]
PHST- 1995/04/20 00:01 [medline]
PHST- 1995/04/20 00:00 [entrez]
AID - S0042-6822(85)71175-0 [pii]
AID - 10.1006/viro.1995.1175 [doi]
PST - ppublish
SO  - Virology. 1995 Apr 20;208(2):450-6. doi: 10.1006/viro.1995.1175.