PMID- 7749560
OWN - NLM
STAT- MEDLINE
DCOM- 19950620
LR  - 20200304
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 3
IP  - 2
DP  - 1995 Mar
TI  - Abnormal integrity of the nucleolus associated with cell cycle arrest owing to 
      the temperature-sensitive ubiquitin-activating enzyme E1.
PG  - 115-23
AB  - A mouse cell mutant, ts85, containing the temperature-sensitive 
      ubiquitin-activating enzyme was arrested in G2 phase at the non-permissive 
      temperature. In the arrested cells, azure C, a nucleolus-specific stain, revealed 
      a U-shaped or ring-shaped arrangement of nucleolar lobes with an unstained region 
      in the center. Silver staining of the nucleolar organizer region (NOR) and 
      fluorescence in situ hybridization (FISH) with rDNA both gave signals in azure 
      C-positive regions. Electron microscopic examination revealed a cloud of 
      unidentified electron-dense particles (diameter approximately 70 nm) in the azure 
      C-negative center space. When the arrested cells were released into M-phase, we 
      observed the association of NOR-bearing chromosomes with a pulverization-like 
      abnormality. FISH with rDNA and NOR silver staining demonstrated that the 
      pulverization-like abnormality was restricted to NORs. The frequent occurrence of 
      persistent nucleolar material in prophase and prometaphase of the stressed cells 
      after release indicated a delayed dissociation of the nucleolus that brought 
      about the abnormal chromosomes in M-phase. ts85 cells transfected with the mouse 
      E1 cDNA recovered growth at the non-permissive temperature and no longer showed 
      abnormal nucleolar morphology. It seems that the ubiquitin system plays a role in 
      the dissolution of the nucleolus, possibly involving the NOR-bearing chromosomes.
FAU - Sudha, T
AU  - Sudha T
AD  - Down's Research Society, Madras, India.
FAU - Tsuji, H
AU  - Tsuji H
FAU - Sameshima, M
AU  - Sameshima M
FAU - Matsuda, Y
AU  - Matsuda Y
FAU - Kaneda, S
AU  - Kaneda S
FAU - Nagai, Y
AU  - Nagai Y
FAU - Yamao, F
AU  - Yamao F
FAU - Seno, T
AU  - Seno T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Ubiquitins)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 6.- (Ligases)
RN  - EC 6.2.1.45 (Ubiquitin-Activating Enzymes)
SB  - IM
MH  - Animals
MH  - Cell Cycle/*physiology
MH  - Cell Division
MH  - Cell Nucleolus/*physiology/ultrastructure
MH  - *Chromosome Aberrations
MH  - DNA, Ribosomal/isolation & purification
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Ligases/*genetics
MH  - Mice
MH  - Microscopy, Electron
MH  - Mutation
MH  - Nucleolus Organizer Region/*physiology/ultrastructure
MH  - Silver Staining
MH  - Tumor Cells, Cultured
MH  - Ubiquitin-Activating Enzymes
MH  - Ubiquitin-Protein Ligases
MH  - Ubiquitins/metabolism
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.1007/BF00710672 [doi]
PST - ppublish
SO  - Chromosome Res. 1995 Mar;3(2):115-23. doi: 10.1007/BF00710672.