PMID- 7749568 OWN - NLM STAT- MEDLINE DCOM- 19950620 LR - 20231213 IS - 0967-3849 (Print) IS - 0967-3849 (Linking) VI - 3 IP - 2 DP - 1995 Mar TI - Localization of seed protein genes on metaphase chromosomes of Vicia faba via fluorescence in situ hybridization. PG - 94-100 AB - Using fluorescence in situ hybridization (FISH), four different seed protein genes were physically mapped on metaphase chromosomes of Vicia faba L. dropped on slides. FISH with a 2.8 kb genomic probe of a legumin B4 gene resulted in reproducible signals on the long arm of chromosome III near the centromere. The same clone cross-hybridized at a lower frequency to the short arm of chromosome II, where the closely related legumin B3 gene family is located. The locus for legumin A-genes could be detected in the distal half of the long arm of chromosome V using a 1.7 kb cDNA clone. The locus of an unknown seed protein gene was mapped to the long arm of chromosome I using a mixture of polymerase chain reaction-amplified DNA fragments of the coding region of up to 1 kb in size. FAU - Fuchs, J AU - Fuchs J AD - Institut fur Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany. FAU - Schubert, I AU - Schubert I LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Chromosome Res JT - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology JID - 9313452 RN - 0 (Plant Proteins) SB - IM MH - *Chromosome Mapping MH - Chromosomes/genetics/ultrastructure MH - Fabaceae/*genetics MH - Genes, Plant/*genetics MH - In Situ Hybridization, Fluorescence MH - Karyotyping MH - Plant Proteins/*genetics MH - *Plants, Medicinal MH - Seeds/*chemistry MH - Legumins EDAT- 1995/03/01 00:00 MHDA- 1995/03/01 00:01 CRDT- 1995/03/01 00:00 PHST- 1995/03/01 00:00 [pubmed] PHST- 1995/03/01 00:01 [medline] PHST- 1995/03/01 00:00 [entrez] AID - 10.1007/BF00710669 [doi] PST - ppublish SO - Chromosome Res. 1995 Mar;3(2):94-100. doi: 10.1007/BF00710669.