PMID- 7750503
OWN - NLM
STAT- MEDLINE
DCOM- 19950619
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 136
IP  - 6
DP  - 1995 Jun
TI  - A potential transcriptional adaptor(s) may be required in thyroid 
      hormone-stimulated gene transcription in vitro.
PG  - 2776-83
AB  - Thyroid hormone (T3) stimulates gene transcription by activation of thyroid 
      hormone receptors (TRs), which bind to thyroid hormone response elements in 
      T3-regulated genes. Retinoid-X receptors (RXRs), major members of the TR 
      auxiliary proteins, have recently been shown to form TR-RXR heterodimers, to 
      enhance the binding of the TRs to thyroid hormone response elements, and to 
      augment TR-mediated transcriptional activation. In this report, we provide 
      evidence that a putative adaptor(s), other than RXR, may be involved in 
      T3-stimulated gene transcription. First, T3-stimulated, but not basal, 
      transcription from the rat GH promoter was progressively and specifically 
      inhibited by the addition of increasing amounts of either GST-TR beta or GST-RXR 
      beta in a cell-free in vitro transcription. Second, this specific transcriptional 
      inhibition is not due to disruption of the DNA-binding activity of the endogenous 
      TR-RXR complex. These results suggest that inhibition of T3-stimulated 
      transcription may be due to the sequestration of a limiting adaptor molecule(s). 
      Hence, we hypothesize that a limiting adaptor(s), which may act as a bridging 
      molecule between the TR-RXR complex and the basal transcriptional machinery, may 
      be sequestered by either GST-TR beta or GST-RXR beta, resulting in the inhibition 
      of T3-stimulated transcription.
FAU - Suen, C S
AU  - Suen CS
AD  - Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 
      02115, USA.
FAU - Chin, W W
AU  - Chin WW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Receptors, Thyroid Hormone)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - 9002-72-6 (Growth Hormone)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Glutathione/pharmacology
MH  - Growth Hormone/genetics
MH  - Humans
MH  - Models, Genetic
MH  - Promoter Regions, Genetic/drug effects
MH  - Rats
MH  - Receptors, Retinoic Acid/metabolism
MH  - Receptors, Thyroid Hormone/metabolism
MH  - Recombinant Fusion Proteins/pharmacology
MH  - Retinoid X Receptors
MH  - Transcription Factors/metabolism
MH  - Transcription, Genetic/*drug effects
MH  - Triiodothyronine/*pharmacology
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - 10.1210/endo.136.6.7750503 [doi]
PST - ppublish
SO  - Endocrinology. 1995 Jun;136(6):2776-83. doi: 10.1210/endo.136.6.7750503.