PMID- 7751599
OWN - NLM
STAT- MEDLINE
DCOM- 19950622
LR  - 20190512
IS  - 0022-1503 (Print)
IS  - 0022-1503 (Linking)
VI  - 86
IP  - 2
DP  - 1995 Mar-Apr
TI  - Characterization of honeybee (Apis mellifera L.) chromosomes using repetitive DNA 
      probes and fluorescence in situ hybridization.
PG  - 145-50
AB  - Two different repetitive DNA probes of Apis mellifera and ribosomal DNA from 
      Drosophila melanogaster were used to characterize the chromosomal set of the 
      honeybee (n = 16). The probes were hybridized to chromosome preparations of 
      haploid testis tissue from drone larvae using fluorescence in situ hybridization 
      (FISH). The honeybee probes hybridized to the telomeric (Alu I family) and 
      centromeric region (Ava I family) of most chromosomes. The rDNA probe labeled two 
      chromosomes only. Combination of the three probes yielded labeled patterns 
      allowing us to identify each chromosome of the honeybee individually. This is the 
      first report of an unambiguous identification of the chromosomal set of the 
      honeybee, since classical banding techniques failed to yield clear patterns for 
      identification. The consensus sequence of the centromeric reiterated probe (Ava I 
      family) has a length of about 550 nucleotides and shows no homology to other 
      known sequences. However, the structural organization of a 130-nucleotides long 
      motif forming the unusually homogeneous 550 nucleotides repeat is similar to 
      those found in mammals' repetitive DNAs.
FAU - Beye, M
AU  - Beye M
AD  - Institut fur Biologie, Technische Universitat Berlin, Germany.
FAU - Moritz, R F
AU  - Moritz RF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Hered
JT  - The Journal of heredity
JID - 0375373
RN  - 0 (DNA Probes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Bees/*genetics
MH  - Centromere
MH  - Chromosomes
MH  - DNA
MH  - *DNA Probes
MH  - Drosophila melanogaster/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Alignment
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.jhered.a111545 [doi]
PST - ppublish
SO  - J Hered. 1995 Mar-Apr;86(2):145-50. doi: 10.1093/oxfordjournals.jhered.a111545.