PMID- 7751730
OWN - NLM
STAT- MEDLINE
DCOM- 19950622
LR  - 20190821
IS  - 0387-5911 (Print)
IS  - 0387-5911 (Linking)
VI  - 69
IP  - 1
DP  - 1995 Jan
TI  - Molecular epidemiology of Staphylococcus spp. contamination in the ward 
      environment: study on mecA and femA genes in methicillin-resistant strains.
PG  - 15-20
AB  - Staphylococcus spp. were isolated from the ward environment and antibiotic 
      susceptibility tests were performed. Twenty-nine strains out of 274 isolates were 
      S. aureus, and 41.4% of the S. aureus strains were methicillin resistant (MRSA). 
      All 12 strains of MRSA were also resistant to oxacillin, ceftizoxime, ampicillin 
      and clindamycin. Among the coagulase-negative staphylococci (CNS), 
      methicillin-resistant (MR) strains of S. epidermidis, S. capitis, S. warneri, S. 
      haemolyticus, S. hominis, S. auricularis, S. saprophyticus and S. cohnii were 
      isolated. Eight of the 10 S. Haemolyticus strains were methicillin resistant. The 
      femA gene was detected in S. aureus (MSSA and MRSA), but not in CNS by polymerase 
      chain reaction (PCR) analysis and Southern blot analysis. The mecA gene was found 
      in all the MRSA and MR-S. epidermidis strains tested, and one of the two MR-S. 
      hominis strains, but not in MSSA, MS-S. epidermidis, MS-S. hominis, or MS-S. 
      haemolyticus. DNA from one strain of MR-S. hominis and 2 strains of MR-S. 
      haemolyticus was not amplified by PCR using the mecA gene primer, or hybridized 
      by Southern blotting. The ambiguity that mecA was detected in some MR-CNS 
      strains, but not in others is discussed.
FAU - Ashimoto, A
AU  - Ashimoto A
AD  - Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Tottori 
      University.
FAU - Hamada, T
AU  - Hamada T
FAU - Adachi, A
AU  - Adachi A
FAU - Tanigawa, T
AU  - Tanigawa T
FAU - Tanaka, Y
AU  - Tanaka Y
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Kansenshogaku Zasshi
JT  - Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious 
      Diseases
JID - 0236671
SB  - IM
GS  - femA
GS  - mecA
MH  - Base Sequence
MH  - *Environmental Microbiology
MH  - Equipment Contamination
MH  - Floors and Floorcoverings
MH  - *Genes, Bacterial
MH  - Methicillin Resistance/*genetics
MH  - Molecular Sequence Data
MH  - Patients' Rooms
MH  - Polymerase Chain Reaction
MH  - Staphylococcus aureus/drug effects/*genetics/isolation & purification
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.11150/kansenshogakuzasshi1970.69.15 [doi]
PST - ppublish
SO  - Kansenshogaku Zasshi. 1995 Jan;69(1):15-20. doi: 
      10.11150/kansenshogakuzasshi1970.69.15.