PMID- 7752500
OWN - NLM
STAT- MEDLINE
DCOM- 19950620
LR  - 20110801
IS  - 0385-2385 (Print)
IS  - 0385-2385 (Linking)
VI  - 37
IP  - 2
DP  - 1995 Feb
TI  - Expression of intercellular adhesion molecule-1 on cultured glomerular 
      endothelial cells by pro-inflammatory cytokines and lipopolysaccharide.
PG  - 100-4
AB  - Infiltration of leukocytes and mononuclear cells into the glomeruli is an early 
      pathological finding in human and experimental glomerulonephritis. However, the 
      cellular and molecular basis for cell infiltration into the glomeruli is not yet 
      completely understood. In addition, there is little information on the expression 
      of intercellular adhesion molecule-1 (ICAM-1) on glomerular cells. In the present 
      study, we investigated the expression of ICAM-1 on cultured bovine glomerular 
      endothelial cells (GEN) and its regulation by the pro-inflammatory cytokines, 
      interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), monocyte chemoattractant 
      protein-1 (MCP-1) and lipopolysaccharide (LPS). Immunocytochemical staining 
      showed that ICAM-1 molecules were constitutively expressed on the surface of GEN. 
      In flow cytometric and ELISA analyses, ICAM-1 molecule expression on GEN was 
      significantly upregulated by IL-1 beta, MCP-1 and LPS in a dose-dependent manner, 
      but not by IL-6. LPS was the most potent inducer of ICAM-1 molecule expression on 
      GEN. The effects of IL-1 beta, MCP-1 and LPS were observed as early as 4 h and 
      reached a maximal level by 18 h. These results suggest that ICAM-1 on GEN can 
      participate in the infiltration of mononuclear cells into glomeruli in human and 
      experimental glomerulonephritis.
FAU - Nitta, K
AU  - Nitta K
AD  - Department of Medicine, Tokyo Women's Medical College, Japan.
FAU - Yumura, W
AU  - Yumura W
FAU - Uchida, K
AU  - Uchida K
FAU - Miwa, N
AU  - Miwa N
FAU - Horita, S
AU  - Horita S
FAU - Tsutsui, T
AU  - Tsutsui T
FAU - Kawashima, A
AU  - Kawashima A
FAU - Nihei, H
AU  - Nihei H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Nihon Jinzo Gakkai Shi
JT  - Nihon Jinzo Gakkai shi
JID - 7505731
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Cytokines/*pharmacology/physiology
MH  - Endothelium/cytology/metabolism
MH  - Glomerulonephritis/metabolism/pathology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/*metabolism
MH  - Kidney Glomerulus/*metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Up-Regulation
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
PST - ppublish
SO  - Nihon Jinzo Gakkai Shi. 1995 Feb;37(2):100-4.