PMID- 7753830
OWN - NLM
STAT- MEDLINE
DCOM- 19950616
LR  - 20220321
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 92
IP  - 10
DP  - 1995 May 9
TI  - Metaphase and interphase fluorescence in situ hybridization mapping of the rice 
      genome with bacterial artificial chromosomes.
PG  - 4487-91
AB  - Fluorescence in situ hybridization (FISH) is a powerful tool for physical mapping 
      in human and other mammalian species. However, application of the FISH technique 
      has been limited in plant species, especially for mapping single- or low-copy DNA 
      sequences, due to inconsistent signal production in plant chromosome 
      preparations. Here we demonstrate that bacterial artificial chromosome (BAC) 
      clones can be mapped readily on rice (Oryza sativa L.) chromosomes by FISH. 
      Repetitive DNA sequences in BAC clones can be suppressed efficiently by using 
      rice genomic DNA as a competitor in the hybridization mixture. BAC clones as 
      small as 40 kb were successfully mapped. To demonstrate the application of the 
      FISH technique in physical mapping of plant genomes, both anonymous BAC clones 
      and clones closely linked to a rice bacterial blight-resistance locus, Xa21, were 
      chosen for analysis. The physical location of Xa21 and the relationships among 
      the linked clones were established, thus demonstrating the utility of FISH in 
      plant genome analysis.
FAU - Jiang, J
AU  - Jiang J
AD  - Department of Plant Pathology, Kansas State University, Manhattan 66506-5502, 
      USA.
FAU - Gill, B S
AU  - Gill BS
FAU - Wang, G L
AU  - Wang GL
FAU - Ronald, P C
AU  - Ronald PC
FAU - Ward, D C
AU  - Ward DC
LA  - eng
GR  - HG-00272/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
SB  - IM
MH  - Animals
MH  - *Chromosomes, Bacterial
MH  - Genetic Techniques
MH  - *Genome, Plant
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Mammals
MH  - Metaphase
MH  - Oryza/*cytology/*genetics
MH  - Repetitive Sequences, Nucleic Acid
PMC - PMC41969
EDAT- 1995/05/09 00:00
MHDA- 1995/05/09 00:01
PMCR- 1995/11/09
CRDT- 1995/05/09 00:00
PHST- 1995/05/09 00:00 [pubmed]
PHST- 1995/05/09 00:01 [medline]
PHST- 1995/05/09 00:00 [entrez]
PHST- 1995/11/09 00:00 [pmc-release]
AID - 10.1073/pnas.92.10.4487 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1995 May 9;92(10):4487-91. doi: 
      10.1073/pnas.92.10.4487.