PMID- 7756251
OWN - NLM
STAT- MEDLINE
DCOM- 19950629
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 34
IP  - 19
DP  - 1995 May 16
TI  - The peptide-binding domain of the chaperone protein Hsc70 has an unusual 
      secondary structure topology.
PG  - 6261-6
AB  - Modern NMR methods were used to determine the secondary structure topology of the 
      18 kDa peptide binding domain of the chaperone protein Hsc70 in solution. This 
      report constitutes the first experimental conformational information on this 
      important domain of the class of Hsp70 proteins. The domain consists of two 
      four-stranded antiparallel beta-sheets and a single alpha-helix. The topology 
      does not resemble at all the topology observed in the human leukocyte antigen 
      (HLA) proteins of the major histocompatibility complex. This is significant 
      because such resemblance was predicted on the basis of limited amino acid 
      homology, secondary structure prediction, and related function. Moreover, the 
      exact meander-type beta-sheet topology identified in Hsc70 has to our best 
      knowledge not been observed in any other known protein structure.
FAU - Morshauser, R C
AU  - Morshauser RC
AD  - Department of Biological Chemistry, University of Michigan, Ann Arbor 48109, USA.
FAU - Wang, H
AU  - Wang H
FAU - Flynn, G C
AU  - Flynn GC
FAU - Zuiderweg, E R
AU  - Zuiderweg ER
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Carrier Proteins)
RN  - 0 (HSC70 Heat-Shock Proteins)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Hspa8 protein, rat)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Carrier Proteins/*chemistry
MH  - HSC70 Heat-Shock Proteins
MH  - *HSP70 Heat-Shock Proteins
MH  - Hydrogen Bonding
MH  - Magnetic Resonance Spectroscopy
MH  - Molecular Chaperones/*chemistry
MH  - Molecular Sequence Data
MH  - Peptides/metabolism
MH  - Protein Structure, Secondary
MH  - Rats
EDAT- 1995/05/16 00:00
MHDA- 1995/05/16 00:01
CRDT- 1995/05/16 00:00
PHST- 1995/05/16 00:00 [pubmed]
PHST- 1995/05/16 00:01 [medline]
PHST- 1995/05/16 00:00 [entrez]
AID - 10.1021/bi00019a001 [doi]
PST - ppublish
SO  - Biochemistry. 1995 May 16;34(19):6261-6. doi: 10.1021/bi00019a001.