PMID- 7756653
OWN - NLM
STAT- MEDLINE
DCOM- 19950623
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 85
IP  - 11
DP  - 1995 Jun 1
TI  - Interphase and metaphase detection of the breakpoint of 14q32 translocations in 
      B-cell malignancies by double-color fluorescence in situ hybridization.
PG  - 3223-8
AB  - The breakpoint of 14q32 translocations found in B-cell malignancies was 
      delineated specifically in both metaphase spreads and interphase nuclei by 
      double-color fluorescence in situ hybridization (FISH) using bacteriophage clones 
      containing the human immunoglobulin gamma chain gene locus (Ig gamma) and a 
      cosmid clone, CY24-68, containing VH segments. CY24-68 is more telomeric than Ig 
      gamma, separated by approximately 1 megabase (Mb). FISH studies were performed on 
      four patients with non-Hodgkin's lymphoma (NHL), one with acute lymphoblastic 
      leukemia (ALL), one with plasma cell leukemia (PCL), and three cell lines. In 
      each patient with t(8;14), t(14;18), and t(3;14), the signal of Ig gamma gene was 
      observed on der(14) and that of CY24-68 at respective partner sites of these 
      translocations, 8q24.1, 18q21.3, and 3q27. Interphase nuclei with a signal of Ig 
      gamma clearly separated from that of CY24-68 were more frequently encountered in 
      all of the patients (45% to 74%) than those in normal controls (4% to 5%). Even 
      in cases where only interphase nuclei were available for FISH studies, 14q32 
      translocations are detected as shown in two patients each with NHL and 
      t(11;14)-carrying PCL. In two cell lines, HS-1 derived from ALL carrying t(8;14) 
      and FR4 derived from a plasmacytoma carrying a complex form of t(8;14), the 
      signal of Ig gamma was observed at the breakpoint region 8q24.1 of the der(8) in 
      addition to the der(14), indicating that translocation event occurred within the 
      Ig gamma locus. Intense Ig gamma signal was found at the breakpoint region on the 
      der(14)t(11;14) in HBL-2 derived from NHL, indicating amplification of the Ig 
      gamma gene, and presumably the resultant chimeric DNA between Ig gamma and DNA 
      sequences at 11q13. The present approach allowed us to unequivocally detect 
      tumor-specific breakpoints of 14q32 translocations. Furthermore, interphase FISH 
      provides a rapid diagnostic procedure to detect 14q32 translocations in B-cell 
      malignancies.
FAU - Taniwaki, M
AU  - Taniwaki M
AD  - Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, 
      Japan.
FAU - Nishida, K
AU  - Nishida K
FAU - Ueda, Y
AU  - Ueda Y
FAU - Misawa, S
AU  - Misawa S
FAU - Nagai, M
AU  - Nagai M
FAU - Tagawa, S
AU  - Tagawa S
FAU - Yamagami, T
AU  - Yamagami T
FAU - Sugiyama, H
AU  - Sugiyama H
FAU - Abe, M
AU  - Abe M
FAU - Fukuhara, S
AU  - Fukuhara S
AU  - et al.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 0 (Immunoglobulin gamma-Chains)
RN  - 0 (Rhodamines)
RN  - 4158-89-8 (tetramethylrhodamine isothiocyanate)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Burkitt Lymphoma/*genetics/pathology
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 14/*ultrastructure
MH  - Chromosomes, Human, Pair 18/ultrastructure
MH  - Chromosomes, Human, Pair 3/ultrastructure
MH  - Chromosomes, Human, Pair 8/ultrastructure
MH  - Cosmids
MH  - Fluorescein-5-isothiocyanate
MH  - Fluorescent Dyes
MH  - *Genes, Immunoglobulin
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - Immunoglobulin Variable Region/genetics
MH  - Immunoglobulin gamma-Chains/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Leukemia, Plasma Cell/*genetics/pathology
MH  - Lymphoma, B-Cell/*genetics/pathology
MH  - Metaphase
MH  - Rhodamines
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - S0006-4971(20)77465-3 [pii]
PST - ppublish
SO  - Blood. 1995 Jun 1;85(11):3223-8.