PMID- 7756862
OWN - NLM
STAT- MEDLINE
DCOM- 19950626
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 78
IP  - 2
DP  - 1995 Mar-Apr
TI  - Determination of malachite green and its metabolite, leucomalachite green, in 
      catfish (Ictalurus punctatus) tissue by liquid chromatography with visible 
      detection.
PG  - 453-7
AB  - To determine residues of malachite green (MG) and its metabolite, leucomalachite 
      green (LMG), in catfish tissue, analytes are extracted with acetonitrile-buffer 
      and the extract is partitioned into methylene chloride. Final cleanup and 
      isolation are performed on neutral alumina solid-phase extraction (SPE) and 
      propylsulfonic acid cation-exchange SPE columns before analysis by liquid 
      chromatography with visible detection. PbO2 postcolumn oxidation is performed by 
      isocratic elution with a buffered mobile phase from a cyano column. Recoveries 
      and relative standard deviations (RSDs) from fortified catfish tissues were 72.9% 
      (RSD, 1.92%; 23 ppb), 75.5% (RSD, 6.85%; 11 ppb), and 69.6% (RSD, 6.93%; 5.7 ppb) 
      for MG and 87.4% (RSD, 2.92%; 21 ppb), 88.1% (RSD, 5.94%; 10 ppb), and 82.6% 
      (RSD, 11.5%; 5.3 ppb) for LMG. The method was applied to MG-incurred catfish at 
      depuration times of 0, 2, 4, 8, and 24 h. Average levels of residual MG and LMG 
      in the 24 h depuration catfish tissue were 73.4 and 289 ppb, respectively.
FAU - Roybal, J E
AU  - Roybal JE
AD  - U.S. Food and Drug Administration, Animal Drugs Research Center, Denver Federal 
      Center, CO 80225-0087, USA.
FAU - Pfenning, A P
AU  - Pfenning AP
FAU - Munns, R K
AU  - Munns RK
FAU - Holland, D C
AU  - Holland DC
FAU - Hurlbut, J A
AU  - Hurlbut JA
FAU - Long, A R
AU  - Long AR
LA  - eng
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Rosaniline Dyes)
RN  - 12058M7ORO (malachite green)
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid/*methods
MH  - *Ictaluridae
MH  - Rosaniline Dyes/*analysis/metabolism
MH  - Sensitivity and Specificity
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 1995 Mar-Apr;78(2):453-7.