PMID- 7758559
OWN - NLM
STAT- MEDLINE
DCOM- 19950623
LR  - 20190821
IS  - 0903-1936 (Print)
IS  - 0903-1936 (Linking)
VI  - 8
IP  - 2
DP  - 1995 Feb
TI  - The effect of prolonged exposure to NO2 from birth on airways responsiveness in 
      rabbits sensitized at birth.
PG  - 246-52
AB  - Our aim was to determine whether daily exposure to 4 ppm nitrogen dioxide (NO2) 
      from birth until 3 months of age influenced the development of airways 
      hyperresponsiveness and atopic sensitivity in immunized rabbits. Littermate New 
      Zealand white (NZW) rabbits were immunized within 24 h of birth by i.p. injection 
      of house dust mite antigen in AI(OH)3 gel, and exposed to either ambient air or 4 
      ppm NO2 for 2 h.day-1, 5 days.week-1. At 3 months, bronchoalveolar lavage (BAL) 
      and serum samples were obtained. Airways responsiveness was measured as the 
      provocative concentrations (mg.ml-1) of histamine or methacholine required to 
      elicit a 50% increase in airway resistance (RLPC50) and a 35% decrease in dynamic 
      compliance (CdynPC35). There were no differences in total cell or differential 
      cell counts recovered in BAL fluid between control and NO2 exposed animals. 
      Airways responsiveness did not differ between groups of animals (histamine RLPC50 
      values: air (n = 15) versus NO2 (n = 13), respectively, 9.98 +/- 1.32 versus 
      16.43 +/- 1.45 mg.ml-1; CdynPC35 values: 16.60 +/- 1.44 versus 14.95 +/- 1.43 
      mg.ml-1; methacholine RLPC50 values: air (n = 14) versus NO2 (n = 12), 
      respectively, 2.18 +/- 1.51 versus 2.21 +/- 1.32 mg.ml-1; CdynPC35 values: 2.64 
      +/- 1.41 versus 2.85 +/- 1.31 mg.ml-1). There was no difference in sensitization 
      between groups of animals exposed to air or NO2, evaluated either by cutaneous 
      responsiveness to intradermal antigen, or serum immunoglobulin E (IgE) levels 
      assessed by the passive cutaneous anaphylaxis (PCA) reaction.(ABSTRACT TRUNCATED 
      AT 250 WORDS)
FAU - Douglas, G J
AU  - Douglas GJ
AD  - Dept of Pharmacology, King's College, University of London, UK.
FAU - Price, J F
AU  - Price JF
FAU - Page, C P
AU  - Page CP
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur Respir J
JT  - The European respiratory journal
JID - 8803460
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - S7G510RUBH (Nitrogen Dioxide)
SB  - IM
MH  - Allergens/immunology
MH  - Animals
MH  - Asthma/*chemically induced/physiopathology
MH  - Bronchial Hyperreactivity/*chemically induced/*physiopathology
MH  - Bronchial Provocation Tests
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Female
MH  - Hypersensitivity, Immediate/*chemically induced/physiopathology
MH  - Immunization
MH  - Immunoglobulin E/immunology
MH  - Male
MH  - Mites/immunology
MH  - Nitrogen Dioxide/*adverse effects
MH  - Passive Cutaneous Anaphylaxis
MH  - Rabbits
MH  - Skin Tests
MH  - Time Factors
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 10.1183/09031936.95.08020246 [doi]
PST - ppublish
SO  - Eur Respir J. 1995 Feb;8(2):246-52. doi: 10.1183/09031936.95.08020246.