PMID- 7758994
OWN - NLM
STAT- MEDLINE
DCOM- 19950628
LR  - 20061115
IS  - 0390-6078 (Print)
IS  - 0390-6078 (Linking)
VI  - 80
IP  - 1
DP  - 1995 Jan-Feb
TI  - Clonality study by fluorescence in situ hybridization of a patient with 
      refractory anemia with ringed sideroblasts and monosomy 7.
PG  - 54-7
AB  - Myelodysplastic syndromes (MDS) are stem cell diseases but it is still 
      controversial whether chromosomal abnormalities occurring in these disorders 
      affect a multipotent stem cell or a committed progenitor. We studied a case of 
      refractory anemia with ringed sideroblasts (RARS) and monosomy 7 in 100% of 
      examined metaphases. Using the fluorescence in situ hybridization (FISH) 
      technique with a probe specific for the centromeric region of chromosome 7, we 
      demonstrated that 15% of BM cells fixed in acetic acid/methanol exhibited a 
      normal diploid karyotype. Applying the FISH technique on PB cells smeared onto a 
      slide, we observed that lymphocytes maintain two chromosomes 7, whereas other 
      leukocytes exhibited monosomy 7. Our study confirms that chromosomal 
      abnormalities found in MDS can occur in cells capable of differentiation along 
      granulocytic and monocytic lineages, but not along the lymphocytic lineage.
FAU - Fugazza, G
AU  - Fugazza G
AD  - Dipartimento di Medicina Interna, Universita di Genova, Italy.
FAU - Lerza, R
AU  - Lerza R
FAU - Bruzzone, R
AU  - Bruzzone R
FAU - Sessarego, M
AU  - Sessarego M
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
SB  - IM
CIN - Haematologica. 1995 Mar-Apr;80(2):95-7. PMID: 7503878
MH  - Anemia, Sideroblastic/*genetics
MH  - *Chromosomes, Human, Pair 7
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - *Monosomy
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PST - ppublish
SO  - Haematologica. 1995 Jan-Feb;80(1):54-7.