PMID- 7762003
OWN - NLM
STAT- MEDLINE
DCOM- 19950629
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 99
IP  - 1-2
DP  - 1995 May 5
TI  - The role of G-proteins in the activation of human leukocytes by particulate 
      stimuli to produce reactive oxygen metabolites.
PG  - 67-76
AB  - Effects of pertussis toxin (PTX), cholera toxin (CTX) and an anhydrolyzable GTP 
      analogue, GTP gamma S, on the levels of free intracellular calcium ([Ca2+]i) and 
      the production of reactive oxygen metabolites (ROM) in human polymorphonuclear 
      leukocytes (PMNL) were studied during cell activation. Cells were stimulated by 
      particulate stimuli, quartz or chrysotile, and soluble stimuli, 
      formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA). 
      Pretreatment of PMNL with PTX decreased fMLP-induced elevations of [Ca2+]i but 
      not those induced by quartz or chrysotile. CTX, in turn, decreased both quartz- 
      and fMLP-induced elevations of [Ca2+]i. Likewise, PTX inhibited only fMLP-induced 
      production of ROM, whereas CTX inhibited also those induced by quartz, chrysotile 
      or fMLP. PTX or CTX did not, however, have an impact on PMA-induced production of 
      ROM. GTP gamma S alone did not elevate [Ca2+]i or amplify fMLP-, quartz- or 
      chrysotile-induced [Ca2+]i elevation. However, GTP gamma S alone increased the 
      production of ROM and amplified ROM production induced by fMLP and quartz. The 
      present results suggest that a CTX-sensitive G-protein may be involved in 
      quartz-induced PMNL activation whereas an fMLP-induced neutrophil activation may 
      be regulated by G-proteins sensitive to both PTX and CTX. The involvement of 
      G-protein in chrysotile-induced leukocyte activation is not likely. There may be, 
      however, a relationship between G-protein-mediated cell signalling and 
      quartz-induced production of reactive oxygen metabolites in these cells.
FAU - Ruotsalainen, M
AU  - Ruotsalainen M
AD  - National Public Health Institute, Department of Toxicology, Kuopio, Finland.
FAU - Savolainen, K M
AU  - Savolainen KM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Asbestos, Serpentine)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 14808-60-7 (Quartz)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9012-63-9 (Cholera Toxin)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Asbestos, Serpentine/pharmacology
MH  - Calcium/metabolism
MH  - Cholera Toxin/pharmacology
MH  - GTP-Binding Proteins/*physiology
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophil Activation/*physiology
MH  - Neutrophils/metabolism
MH  - Pertussis Toxin
MH  - Quartz/pharmacology
MH  - Reactive Oxygen Species/*metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Virulence Factors, Bordetella/pharmacology
EDAT- 1995/05/05 00:00
MHDA- 1995/05/05 00:01
CRDT- 1995/05/05 00:00
PHST- 1995/05/05 00:00 [pubmed]
PHST- 1995/05/05 00:01 [medline]
PHST- 1995/05/05 00:00 [entrez]
AID - 0300-483X(94)02996-8 [pii]
AID - 10.1016/0300-483x(94)02996-8 [doi]
PST - ppublish
SO  - Toxicology. 1995 May 5;99(1-2):67-76. doi: 10.1016/0300-483x(94)02996-8.