PMID- 7762660
OWN - NLM
STAT- MEDLINE
DCOM- 19950629
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 268
IP  - 5 Pt 1
DP  - 1995 May
TI  - Intestinal anaphylaxis: mediation of the response of colonic longitudinal muscle 
      in rat.
PG  - G764-71
AB  - Colonic smooth muscle function may be altered in food protein hypersensitivity 
      reactions and could contribute to the clinical manifestation of diarrhea. To 
      characterize such functional changes and elucidate the mediators and mechanisms 
      involved. Hooded-Lister rats were sensitized by intraperitoneal injection of egg 
      albumin (10 micrograms), and controls were sham sensitized with saline. Fourteen 
      days later the contractility of longitudinally oriented distal colonic segments 
      (mucosa intact) were studied in standard tissue baths in response to antigen (Ag) 
      or other agents. After Ag exposure, a contractile response was documented in 
      animals that were sensitized [specific immunoglobulin E (IgE) antibody levels > 
      or = 1:64] and was specific for the sensitizing Ag. Mast cell involvement was 
      suggested by a significant reduction in the number of granulated mucosal mast 
      cells in sensitized tissues after Ag challenge and in the magnitude of the 
      Ag-induced contractile response in the presence of mast cell stabilizers. The 
      antigen-induced response was significantly and independently inhibited by both 
      cyclooxygenase and lipoxygenase enzyme inhibitors and by leukotriene D4 and 
      platelet activating factor receptor antagonists. The Ag-induced response was 
      resistant to histamine and the 5-hydroxytryptamine antagonists, atropine and 
      tetrodotoxin. These results suggest that the food protein-induced contraction of 
      colonic longitudinal smooth muscle in the sensitized rat is due to IgE-mediated 
      mast cell activation with the subsequent production and release of 
      membrane-derived mediators that, in vitro, act directly on the smooth muscle.
FAU - Oliver, M R
AU  - Oliver MR
AD  - Gastrointestinal Research Group, University of Calgary, Alberta, Canada.
FAU - Tan, D T
AU  - Tan DT
FAU - Scott, R B
AU  - Scott RB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Albumins)
RN  - 0 (Antigens)
RN  - 0 (Histamine Antagonists)
RN  - 0 (Serotonin Antagonists)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Albumins/immunology
MH  - Anaphylaxis/*physiopathology
MH  - Animals
MH  - Antigens/immunology
MH  - Colon/*physiopathology
MH  - Eggs
MH  - Gastrointestinal Motility/drug effects
MH  - Histamine Antagonists/pharmacology
MH  - Immunoglobulin E/physiology
MH  - Intestines/*immunology
MH  - Mast Cells/physiology
MH  - Muscle, Smooth/*physiopathology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Serotonin Antagonists/pharmacology
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - 10.1152/ajpgi.1995.268.5.G764 [doi]
PST - ppublish
SO  - Am J Physiol. 1995 May;268(5 Pt 1):G764-71. doi: 10.1152/ajpgi.1995.268.5.G764.