PMID- 7762684
OWN - NLM
STAT- MEDLINE
DCOM- 19950629
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 268
IP  - 5 Pt 1
DP  - 1995 May
TI  - Interleukin-8 and monocyte chemoattractant protein-1 mRNAs in oxygen-injured 
      rabbit lung.
PG  - L826-31
AB  - The chemokines interleukin-8 (IL-8) and monocyte chemoattractant protein-1 
      (MCP-1) are neutrophil and monocyte attractants, respectively. We hypothesized 
      that IL-8 and MCP-1 mRNA expression in alveolar macrophages (AM) lavaged from 
      rabbit lung would be increased by oxygen exposure, which is known to induce 
      inflammation. Adult rabbits were exposed to > 95% oxygen for up to 64 h and 
      allowed to recover in room air for up to 72 h before killing and pulmonary 
      lavage. Numbers of lavageable polymorphonuclear cells (PMN) and AM rose during 
      the exposure protocol. Quantitative in situ hybridization with 3H-labeled cRNA 
      probes showed both IL-8 and MCP-1 mRNA expression in AM during oxygen exposure, 
      with peak levels of IL-8 mRNA at 56-h oxygen exposure and of MCP-1 mRNA at 64-h 
      oxygen exposure with 24-h room air recovery. IL-8 mRNA was present in PMN between 
      48-h oxygen exposure and 64-h oxygen exposure with 24-h room air recovery. MCP-1 
      mRNA was not expressed in PMN. This pattern of chemokine mRNA expression 
      emphasizes the importance of inflammatory cells as effectors in the pulmonary 
      response to oxygen exposure.
FAU - D'Angio, C T
AU  - D'Angio CT
AD  - Department of Pediatrics/Neonatology, Strong Children's Research Center, 
      University of Rochester, New York 14642, USA.
FAU - Sinkin, R A
AU  - Sinkin RA
FAU - LoMonaco, M B
AU  - LoMonaco MB
FAU - Finkelstein, J N
AU  - Finkelstein JN
LA  - eng
GR  - CA-27791/CA/NCI NIH HHS/United States
GR  - HL-07216/HL/NHLBI NIH HHS/United States
GR  - HL-36543/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*genetics
MH  - Cytokines/genetics
MH  - Interleukin-8/*genetics
MH  - Lung/*drug effects/*metabolism/pathology
MH  - Macrophages, Alveolar/metabolism
MH  - Male
MH  - Neutrophils/metabolism
MH  - Oxygen/*poisoning
MH  - RNA, Messenger/*metabolism
MH  - Rabbits
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - 10.1152/ajplung.1995.268.5.L826 [doi]
PST - ppublish
SO  - Am J Physiol. 1995 May;268(5 Pt 1):L826-31. doi: 10.1152/ajplung.1995.268.5.L826.