PMID- 7762688
OWN - NLM
STAT- MEDLINE
DCOM- 19950629
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 268
IP  - 5 Pt 1
DP  - 1995 May
TI  - Tannin inhibits adenylate cyclase in airway epithelial cells.
PG  - L851-5
AB  - Tannin, isolated from cotton bracts extract and implicated in the pathogenesis of 
      byssinosis, inhibits adenosine 3',5'-cyclic monophosphate (cAMP) production and 
      Cl- secretion in bovine airway epithelial cells in part by inhibiting adrenergic 
      receptor binding. The purpose of this study was to determine whether tannin 
      affected other parts of the adrenergic-cAMP signal transduction pathway by 
      examining the effect of tannin on guanosine 5'-triphosphate (GTP)-regulatory 
      pathways (G proteins) and on adenylate cyclase activity. cAMP production in 
      confluent airway epithelial cells was measured in the presence of cholera toxin 
      (100 micrograms/ml), an activator of GS proteins, and forskolin (0.1-1,000 
      microM), a direct activator of adenylate cyclase. Cholera toxin stimulated cAMP 
      production; this response, however, was inhibited in cells pretreated with 50 
      micrograms/ml tannin. Forskolin (100 microM) stimulated cAMP production 13-fold 
      above baseline values. Tannin pretreatment inhibited the stimulatory effect of 
      forskolin on cAMP release in a dose-dependent manner with a tannin concentration 
      causing 50% inhibition of 7.5 micrograms/ml. The stimulatory effect of forskolin 
      on cAMP release was completely inhibited in cells pretreated with 50 
      micrograms/ml tannin. The inhibition was reversible 3 h after removal of tannin 
      from the solution. Tannin also inhibited forskolin-stimulated adenylate cyclase 
      activity in a dose-dependent, noncompetitive manner. We conclude that forskolin 
      and cholera toxin stimulate cAMP production in airway epithelial cells and that 
      tannin inhibits the production of cAMP in airway epithelial cells by a direct 
      effect on adenylate cyclase activity.
FAU - Cloutier, M M
AU  - Cloutier MM
AD  - Pediatric Pulmonary Division, University of Connecticut Health Center, Farmington 
      06030, USA.
FAU - Guernsey, L
AU  - Guernsey L
LA  - eng
GR  - HL-28669/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Adenylyl Cyclase Inhibitors)
RN  - 0 (Hydrolyzable Tannins)
RN  - 1F7A44V6OU (Colforsin)
RN  - 9012-63-9 (Cholera Toxin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - *Adenylyl Cyclase Inhibitors
MH  - Adenylyl Cyclases/metabolism
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Cholera Toxin/pharmacology
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Hydrolyzable Tannins/*pharmacology
MH  - Intracellular Membranes/metabolism
MH  - Trachea/cytology/*enzymology
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - 10.1152/ajplung.1995.268.5.L851 [doi]
PST - ppublish
SO  - Am J Physiol. 1995 May;268(5 Pt 1):L851-5. doi: 10.1152/ajplung.1995.268.5.L851.