PMID- 7764009
OWN - NLM
STAT- MEDLINE
DCOM- 19931014
LR  - 20191031
IS  - 0141-0229 (Print)
IS  - 0141-0229 (Linking)
VI  - 15
IP  - 9
DP  - 1993 Sep
TI  - Detergent permeabilized yeast cells as the source of intracellular enzymes for 
      estimation of biomolecules.
PG  - 796-800
AB  - The feasibility of using permeabilized whole cells as a source of intracellular 
      enzymes instead of isolated expensive enzymes for the estimation of biomolecules 
      has been studied. Alcohol dehydrogenase (ADH), glucose-6-phosphate dehydrogenase 
      (G6PDH), hexokinase (HK), and beta-galactosidase (beta-GAL) activities of 
      cetyltrimethylammonium bromide (CTAB)-permeabilized whole yeast cells were 
      employed to estimate ethyl alcohol, glucose, and lactose. The method using 
      permeabilized cells was comparable to that of isolated enzymes and was applicable 
      for the estimation of these analytes in complex samples such as blood, milk, and 
      fermented samples. The usefulness of permeabilized cells as a single source of 
      more than one enzyme required for coupled enzyme assays was demonstrated.
FAU - Bhat, N
AU  - Bhat N
AD  - Department of Food Chemistry, Central Food Technological Research Institute, 
      Mysore, India.
FAU - Naina, N S
AU  - Naina NS
FAU - Gowda, L R
AU  - Gowda LR
FAU - Bhat, S G
AU  - Bhat SG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Enzyme Microb Technol
JT  - Enzyme and microbial technology
JID - 8003761
RN  - 0 (Cetrimonium Compounds)
RN  - 0 (Detergents)
RN  - 3K9958V90M (Ethanol)
RN  - EC 1.1.1.1 (Alcohol Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 3.2.1.23 (beta-Galactosidase)
RN  - IY9XDZ35W2 (Glucose)
RN  - J2B2A4N98G (Lactose)
RN  - Z7FF1XKL7A (Cetrimonium)
MH  - Alcohol Dehydrogenase/metabolism
MH  - Biological Assay/methods
MH  - Cell Membrane Permeability/drug effects
MH  - Cetrimonium
MH  - Cetrimonium Compounds/pharmacology
MH  - Detergents/pharmacology
MH  - Ethanol/*analysis
MH  - Glucose/*analysis
MH  - Glucosephosphate Dehydrogenase/metabolism
MH  - Hexokinase/metabolism
MH  - Kluyveromyces/enzymology
MH  - Lactose/*analysis
MH  - Saccharomyces cerevisiae/enzymology
MH  - Yeasts/*enzymology
MH  - beta-Galactosidase/metabolism
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 0141-0229(93)90012-Q [pii]
AID - 10.1016/0141-0229(93)90012-q [doi]
PST - ppublish
SO  - Enzyme Microb Technol. 1993 Sep;15(9):796-800. doi: 10.1016/0141-0229(93)90012-q.