PMID- 7766709
OWN - NLM
STAT- MEDLINE
DCOM- 19950703
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1266
IP  - 3
DP  - 1995 May 12
TI  - Molecular attributes of bovine aortic endothelial cell heparan sulfate.
PG  - 235-44
AB  - Heparan sulfate (HS) secreted into the medium of bovine aortic endothelial cell 
      (BAEC) cultures was subjected to chemical and enzymatic degradation followed by 
      analysis using gel-filtration and ion-exchange chromatography. Treatment with 
      HNO2 showed that 41% of the disaccharides were N-sulfated. Degradation by Heparin 
      lyases I (Hep I) showed that 8-9% of the disaccharides contained IdoA(2S) 
      residues. Heparin lyase III (Hep III) degradation produced mainly disaccharides 
      with 67% of the molecules glycosidic linkages susceptible to cleavage. Further 
      degradation of Hep III-resistant fragments with Hep I showed that IdoA(2S) 
      residues were predominantly positioned centrally within the repeating GlcNSO3(+/- 
      6S)alpha 1-4IdoA containing domains. Digestion with a mixture of Heparin lyases 
      I, II and III degraded the molecule almost entirely to disaccharides, with small 
      amounts of tetrasaccharides containing resistant linkages, suggesting the 
      presence of 3-O sulfated GlcNSO3. Further analysis of the disaccharide products 
      by ion-exchange chromatography and comparison with the data from single enzymatic 
      digestion, allowed an estimate of the disaccharide composition to be made. The 
      results suggest an ordered arrangement of structural domains; however, variations 
      in the structure of these domains results in a heterogeneous population of HS 
      chains. It is suggested that biosynthetic differences in HS structure may act as 
      a regulator of bFGF induced cellular responses.
FAU - Pye, D A
AU  - Pye DA
AD  - Department of Clinical Research, Christie Hospital, Manchester, United Kingdom.
FAU - Kumar, S
AU  - Kumar S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Disaccharides)
RN  - 0 (Oligosaccharides)
RN  - 3402-98-0 (Iduronic Acid)
RN  - 9050-30-0 (Heparitin Sulfate)
RN  - EC 4.2.2.- (Polysaccharide-Lyases)
RN  - EC 4.2.2.7 (Heparin Lyase)
SB  - IM
MH  - Animals
MH  - Aorta
MH  - Carbohydrate Sequence
MH  - Cattle
MH  - Cells, Cultured
MH  - Chromatography, Gel
MH  - Chromatography, Ion Exchange
MH  - Disaccharides/isolation & purification
MH  - Endothelium, Vascular/*chemistry
MH  - Heparin Lyase
MH  - Heparitin Sulfate/*chemistry/isolation & purification
MH  - Iduronic Acid/analysis
MH  - Molecular Sequence Data
MH  - Oligosaccharides/isolation & purification
MH  - Polysaccharide-Lyases
EDAT- 1995/05/12 00:00
MHDA- 1995/05/12 00:01
CRDT- 1995/05/12 00:00
PHST- 1995/05/12 00:00 [pubmed]
PHST- 1995/05/12 00:01 [medline]
PHST- 1995/05/12 00:00 [entrez]
AID - 0167-4889(95)00012-H [pii]
AID - 10.1016/0167-4889(95)00012-h [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1995 May 12;1266(3):235-44. doi: 
      10.1016/0167-4889(95)00012-h.