PMID- 7766723
OWN - NLM
STAT- MEDLINE
DCOM- 19950706
LR  - 20190815
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 13
IP  - 2
DP  - 1995 Feb
TI  - Determination of ticarcillin in human plasma and in urine by reversed-phase LC.
PG  - 159-64
AB  - A liquid chromatographic assay for ticarcillin (ticar.) in plasma and urine is 
      described. For analysis, the internal standard cefoperazone (cfp) is dissolved in 
      acetonitrile, which is used for precipitating the protein. The supernatant is 
      evaporated, reconstituted in running mobile phase and injected directly onto the 
      reversed-phase C18 column, with detection at 205 nm. The mobile phase is composed 
      of water-acetonitrile-o-phosphoric acid-tetramethylammonium chloride (TMA). 
      Coefficients of variation for reproducibility were in the range of 2.2-15.5% for 
      extra-low, low, medium and high controls. Limits of detection were 0.5 microgram 
      ml-1 for plasma and 1 microgram ml-1 for urine. No interference from other 
      cephalosporins or other antibiotics was noted. This liquid chromatographic assay 
      is simple, accurate, requires no extraction and overcomes previous problems 
      related to the drug's peak splitting due to isomerization.
FAU - La Follette, G
AU  - La Follette G
AD  - Division of Clinical Pharmacy, School of Pharmacy, University of California, San 
      Francisco 94143-0622, USA.
FAU - Jayewardene, A L
AU  - Jayewardene AL
FAU - Seneviratne, A K
AU  - Seneviratne AK
FAU - Lin, E T
AU  - Lin ET
FAU - Gambertoglio, J G
AU  - Gambertoglio JG
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - F93UJX4SWT (Ticarcillin)
SB  - IM
MH  - Calibration
MH  - Chromatography, Liquid/methods
MH  - Drug Stability
MH  - Humans
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Ticarcillin/*blood/*urine
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 0731-7085(94)00137-Q [pii]
AID - 10.1016/0731-7085(94)00137-q [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 1995 Feb;13(2):159-64. doi: 10.1016/0731-7085(94)00137-q.