PMID- 7769266
OWN - NLM
STAT- MEDLINE
DCOM- 19950706
LR  - 20190723
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 104
IP  - 6
DP  - 1995 Jun
TI  - Comparative study of hepatocyte growth factor/scatter factor and keratinocyte 
      growth factor effects on human keratinocytes.
PG  - 958-63
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) and keratinocyte growth factor 
      (KGF, also designated FGF-7) are paracrine growth factors secreted by mesenchymal 
      cells and active on a variety of epithelial cell types. In this study, the 
      biologic responses of keratinocytes to these paracrine growth factors were 
      compared. Stimulation of mitogenesis, migration, plasminogen activator (PA) 
      activity, and fibronectin production were examined using human foreskin 
      keratinocytes cultured in serum-free MCDB 153 medium. Although the two factors 
      stimulated a similar level of proliferation when cells were maintained for 5 d in 
      1.8 mM Ca++, the peak effect of KGF, observed at 10 ng/ml, was approximately 
      threefold higher than that of HGF/SF when cells were in medium containing 0.15 mM 
      Ca++. Both agents promoted the migration of cells in low-calcium medium (0.08 mM 
      Ca++). However, the magnitude of the response was approximately twofold greater 
      for HGF/SF at 10 ng/ml than KGF at the same concentration. None of the matrix 
      proteins such as type I collagen, type IV collagen, laminin, or fibronectin 
      either stimulated or suppressed HGF/SF- or KGF-stimulated keratinocyte migration. 
      Both factors stimulated PA activity of the cell extracts, especially 
      urokinase-type, with similar potencies. Promoted PA activity was maximal with the 
      addition of 10 ng/ml of either factor. Neither factor increased the production of 
      fibronectin under conditions in which transforming growth factor-beta 1 was 
      active. These results indicate that HGF/SF and KGF, both recognized as paracrine 
      growth factors, elicit distinctive patterns of response by keratinocytes, 
      implying that they have different roles in epidermal physiology.
FAU - Sato, C
AU  - Sato C
AD  - Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan.
FAU - Tsuboi, R
AU  - Tsuboi R
FAU - Shi, C M
AU  - Shi CM
FAU - Rubin, J S
AU  - Rubin JS
FAU - Ogawa, H
AU  - Ogawa H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (FGF7 protein, human)
RN  - 0 (Fibroblast Growth Factor 10)
RN  - 0 (Fibronectins)
RN  - 0 (Growth Substances)
RN  - 126469-10-1 (Fibroblast Growth Factor 7)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 3.4.21.- (Plasminogen Activators)
SB  - IM
MH  - Cell Movement/drug effects
MH  - Fibroblast Growth Factor 10
MH  - Fibroblast Growth Factor 7
MH  - *Fibroblast Growth Factors
MH  - Fibronectins/biosynthesis
MH  - Growth Substances/*pharmacology
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Keratinocytes/cytology/*drug effects/physiology
MH  - Plasminogen Activators/pharmacology
EDAT- 1995/06/01 00:00
MHDA- 1995/06/01 00:01
CRDT- 1995/06/01 00:00
PHST- 1995/06/01 00:00 [pubmed]
PHST- 1995/06/01 00:01 [medline]
PHST- 1995/06/01 00:00 [entrez]
AID - S0022-202X(15)42203-1 [pii]
AID - 10.1111/1523-1747.ep12606221 [doi]
PST - ppublish
SO  - J Invest Dermatol. 1995 Jun;104(6):958-63. doi: 10.1111/1523-1747.ep12606221.