PMID- 7769849
OWN - NLM
STAT- MEDLINE
DCOM- 19950706
LR  - 20240104
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 9
IP  - 5
DP  - 1995 May
TI  - Extensive amplification of bcr/abl fusion genes clustered on three marker 
      chromosomes in human leukemic cell line K-562.
PG  - 858-62
AB  - Using fluorescence in situ hybridization (FISH), we were able to demonstrate 
      22-24-fold amplification of the bcr/abl fusion gene in the human leukemic cell 
      line K-562. About 60% of the amplified sequences are localized to a large 
      acrocentric marker chromosome, with another 30% clustered on a small acrocentric 
      chromosome. In addition to these two masked Ph chromosomes, the remaining bcr/abl 
      fusion genes are located on a der(2) distal to band q33. G- and C-banding 
      analysis revealed similar unique banding patterns in both masked Ph chromosomes 
      and suggests that amplification occurred by tandem duplication of the bcr/abl 
      fusion site. Because the number of bcr/abl fusion genes may be increasing over 
      time, it is critical that researchers using K-562 cells should be aware of this 
      extensive amplification.
FAU - Wu, S Q
AU  - Wu SQ
AD  - Wisconsin Comprehensive Cancer Center, Madison, USA.
FAU - Voelkerding, K V
AU  - Voelkerding KV
FAU - Sabatini, L
AU  - Sabatini L
FAU - Chen, X R
AU  - Chen XR
FAU - Huang, J
AU  - Huang J
FAU - Meisner, L F
AU  - Meisner LF
LA  - eng
GR  - CA14520/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (DNA, Neoplasm)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
GS  - bcr
GS  - bcr/abl
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 22
MH  - *Chromosomes, Human, Pair 9
MH  - DNA, Neoplasm/genetics
MH  - Evaluation Studies as Topic
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - *Gene Amplification
MH  - *Genes, abl
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/physiology
MH  - Karyotyping
MH  - Metaphase/physiology
MH  - Multigene Family
MH  - *Philadelphia Chromosome
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1995 May;9(5):858-62.