PMID- 7771065
OWN - NLM
STAT- MEDLINE
DCOM- 19950706
LR  - 20220316
IS  - 0300-9858 (Print)
IS  - 0300-9858 (Linking)
VI  - 32
IP  - 2
DP  - 1995 Mar
TI  - Immunohistochemical identification of porcine reproductive and respiratory 
      syndrome virus (PRRSV) antigen in the heart and lymphoid system of three-week-old 
      colostrum-deprived pigs.
PG  - 200-4
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) antigens were 
      detected by a streptavidin-biotin complex technique in tissues of 3-week-old 
      colostrum-deprived pigs that had been inoculated intranasally with PRRSV and had 
      developed moderate respiratory disease. Moderate, multifocal, tan-colored 
      consolidation of the lungs and severe enlargement of the lymph nodes were noted 
      at necropsy. Severe interstitial pneumonia characterized by type 2 pneumocyte 
      proliferation, septal infiltration with mononuclear cells, and accumulation of 
      macrophages and necrotic cells in alveolar spaces was observed at 4 and 9 days 
      postinoculation. Moderate multifocal perivascular lymphohistiocytic myocarditis 
      was observed at 9 days postinoculation. Marked lymphoid follicular hyperplasia 
      and follicular necrosis in the tonsil, spleen, and lymph nodes was observed. A 
      monoclonal antibody that recognizes a conserved epitope of PRRSV nucleocapsid 
      protein was used as primary antibody for immunohistochemistry. Antigen was 
      readily detected in alveolar macrophages in the lung and in endothelial cells and 
      macrophages in the heart. Macrophages and cells resembling dendritic cells in 
      tonsil, lymph nodes, thymus, and spleen also stained intensely positive for viral 
      antigen. PRRSV appears to replicate primarily within macrophages in the 
      respiratory and lymphoid systems of the pig.
FAU - Halbur, P G
AU  - Halbur PG
AD  - Iowa State University, Veterinary Medical Research Institute, Ames 50011, USA.
FAU - Miller, L D
AU  - Miller LD
FAU - Paul, P S
AU  - Paul PS
FAU - Meng, X J
AU  - Meng XJ
FAU - Huffman, E L
AU  - Huffman EL
FAU - Andrews, J J
AU  - Andrews JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Vet Pathol
JT  - Veterinary pathology
JID - 0312020
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antigens, Viral/*analysis
MH  - Arterivirus/*immunology
MH  - Colostrum
MH  - Female
MH  - Food Deprivation
MH  - Immunoenzyme Techniques/veterinary
MH  - Infertility, Female/immunology/pathology/*veterinary/virology
MH  - Lung Diseases/immunology/pathology/*veterinary/virology
MH  - Lymphoid Tissue/immunology/pathology
MH  - Myocardium/immunology/pathology
MH  - Swine
MH  - Swine Diseases/immunology/pathology/*virology
MH  - Syndrome
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - 10.1177/030098589503200218 [doi]
PST - ppublish
SO  - Vet Pathol. 1995 Mar;32(2):200-4. doi: 10.1177/030098589503200218.